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<strong>Column</strong>s for Bioanalytical ChromatographyLong Life at High pH with 300Extend-C18<strong>Column</strong>: ZORBAX Extend-C18773450-9024.6 x 150 mm, 5 µmMobile Phase: 20% 20 mM NH 4 OH, pH 10.580% Methanol864-Chloro-1-nitrobenzeneTrimipramineFlow Rate: 1.5 mL/minTemperature: Aging 24°CTests 40°CEach 10,000 column volume is approximately oneworking month.k Values4200 10,000 20,000 30,000 40,000 50,000<strong>Column</strong> Volumes of Mobile PhaseLC30001Extend-C18 and StableBond SB-C18 Are Stable at Low pH<strong>Column</strong> A: ZORBAX SB-C18883975-9024.6 x 150 mm, 5 µm<strong>Column</strong> B: ZORBAX Extend-C18773450-9024.6 x 150 mm, 5 µm<strong>Column</strong> C: ZORBAX Rx-C18883967-9024.6 x 150 mm, 5 µmMobile Phase: <strong>Column</strong> Aging:50% Methanol :50% Water + 1% TFA<strong>Column</strong> Test:60% Methanol : 40% WaterTest Solute: TolueneFlow Rate: 1.5 mL/min, continuousTemperature: Aging: 90°CTest: AmbientThe 300Extend-C18 column can be used at high andlow pH – from pH 2-11.5. This chart shows that the300Extend-C18 has the needed stability at low pH forlong-term reproducible separations. Therefore, onewide-pore column can be used for selectivity optimizationat low and high pH with both TFA and ammoniumhydroxide mobile phases.Fraction of Toluene k Remaining1.00.80.60.40.20.0Stablebond SB-C18 (Diisobutyl-C18)Extend-C18 (Bidentate-C18)Rx-C18 (Dimethyl-C18)0 5,000 10,000 15,000 20,000 25,000 30,000<strong>Column</strong> Volumes of Mobile PhaseLC30002Order online at www.agilent.com/chem/store119
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