Agilent HPLC Column Selection Guide

Columns for Bioanalytical ChromatographyZORBAX 300Å Extend-C18• Rugged, high and low pH separations of polypeptides and peptides from pH 2-11.5• Different selectivity possible at high and low pH• High efficiency and good recovery of hydrophobic peptides at high pH• Ideal for LC/MS with ammonium hydroxide-modified mobile phaseAgilent ZORBAX 300Extend C-18 is a wide-pore HPLC column for high efficiency separationsof peptides from pH 2-11.5. The unique, bidentate bonded phase provides excellent lifetimeand reproducibility at high and low pH. At high pH, retention and selectivity of peptides andpolypeptides can change dramatically as a result of changes in charge on molecules. Excellentrecoveries of hydrophobic polypeptides have been achieved at room temperature and highpH. LC/MS sensitivity of peptides and polypeptides can also be improved at high pH using asimple ammonium hydroxide-containing mobile phase.Novel Bidentate C18-C18 Bonding for Extend-C18Bonded PhaseColumn SpecificationsBonded PhasePoreSizeSurfaceAreaTemp.Limits* pH Range EndcappedCarbonLoadZORBAX 300Extend-C18 300Å 45 m 2 /g 60°C 2.0-11.5 Double 4%Specifications represent typical values only.*Temperature limits are 60°C up to pH 8, 40°C from pH 8-11.5.118 Order online at www.agilent.com/chem/store