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<strong>Column</strong>s for Bioanalytical ChromatographyMicroBore Poroshell 300 <strong>Column</strong>sProvide Maximum Sensitivity for LC/MS<strong>Column</strong>: Poroshell 300SB-C18661750-9021.0 x 75 mm, 5 µmMobile Phase: A: Water + 0.1% Formic AcidB: ACN + 0.1% Formic Acid1EBFlow Rate: 600 µL/minGradient: 20-100% B in 5.5 min 80000000Temperature: 80°CMS Conditions: LC/MS: Pos. Ion ESI –Vcap 6000 V60000000Drying Gas Flow: 12 Liters/minDrying Gas Temperature: 350°CNebulizer: 45 psiFragmentor Volatage: 140 V40000000Scan: 600-2500Stepsize: 0.15 amuPeakwidth: 0.06 min20000000Sample: 1 µL0.50.751.02.55.00 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0Time (min)LCPO003With narrow bore diameters like 2.1 mm, 1.0 mm, and 0.5 mm, ZORBAX Poroshell columns make an ideal LC/MS partner. When the sample is very limited, the 1.0 mmor 0.5 mm ID Poroshell columns are an excellent choice for high sensitivity LC/MS analyses. Sensitive MS molecular weight determinations are possible with as little as0.5 to 5 pmole of protein on Poroshell columns. Poroshell columns have also been used for rapid MS identification of intact proteins even in the presence of stabilizers andtissue culture media.Poroshell 300Hardware Description Size (mm) Particle Size (µm)Poroshell300SB-C18Poroshell300SB-C8Poroshell300SB-C3Poroshell300Extend-C18Narrow Bore 2.1 x 75 5 660750-902 660750-906 660750-909 670750-902MicroBore 1.0 x 75 5 661750-902 661750-906 661750-909 671750-902Capillary 0.5 x 75 5 5065-4468Guard Cartridge, 4/pk 2.1 x 12.5 5 821075-920 821075-918 821075-924Guard Hardware Kit 820888-901 820888-901 820888-901MicroBore Guard, 3/pk 1.0 x 17 5 5185-5968 5185-5968 5185-5968 5185-5968112 Order online at www.agilent.com/chem/store
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