Agilent HPLC Column Selection Guide

Columns for Bioanalytical ChromatographyPoroshell 300 Columns Can SeparateProteins and Peptides in SecondsColumn: Poroshell 300SB-C18660750-9022.1 x 75 mm, 5 µmMobile Phase: A: 0.1% TFA in H 2 0B: 0.07% TFA in ACNFlow Rate: 3.0 mL/minGradient: 5-100% B in 1.0 minTemperature: 70°C, 260 bar pressureDetector: 215 nmSample:Proteins and Peptides1. Angiotensin II2. Neurotensin3. RNase4. Insulin5. Lysozyme6. Myoglobin7. Carbonic Anhydrase8. Ovabumin12345678This separation of eight polypeptides and proteins iscompleted in less than 60 seconds. Each peak is sharpand efficient.0 0.51.0Time (min)LCPO001Reduce Peptide Map Analysis Timeby 90% with Poroshell 300SBColumn A: Poroshell 300SB-C18660750-9022.1 x 75 mm, 5 µmColumn B: ZORBAX 300SB-C18883750-9022.1 x 150 mm, 5 µmMobile Phase: A: 95% H 2 O, 5% ACN, 0.1% TFAB: 5% H 2 O, 95% ACN, 0.07% TFAFlow Rate: 1 mL/min0.208 mL/minGradient: 0-100%B = 12 min0-100%B = 120 minTemperature: 70°CSample:20 µL (0.22 µg/1 µL)BSA Tryptic Digest(15 hours, 70 pmol)AB1 2 3 4 5 6 7 8Time (min)A single chromatographic run of a protein tryptic digest can require an houror more to complete. With ZORBAX Poroshell columns, the same complexseparation can be completed in 1/10th the time.1020 30 40 50 60 70 80Time (min)LCPO002Order online at www.agilent.com/chem/store111