Agilent HPLC Column Selection Guide

Columns for Bioanalytical ChromatographyPorous ShellPoroshell 300SOLIDCORE5 µm• High-resolution separations of biomolecules with superficially porous particles• High efficiency and recovery with proteins (up to 1,000 kDa) and monoclonal antibodies• Achieve long lifetime at low pH with Poroshell 300SB; at high pH with 300Extend-C18• Optimize recovery and selectivity with four different bonded phases – 300SB-C18,300SB-C8, 300SB-C3, and 300Extend-C18Agilent Poroshell 300 columns are ideal for fast separations of proteins and peptides becausethe superficially porous particle allows for fast flow rates to be used while maintaining sharp,efficient peaks. Peptides and proteins are typically separated slowly to reduce the potentialpeak broadening of these slow diffusing analytes. But Poroshell columns use a superficiallyporous particle made with a thin layer of porous silica on a solid core of silica. This reducesthe diffusion distance for proteins making practical rapid HPLC separations of peptides andproteins up to 500-1,000 kDA. Poroshell columns bonded with StableBond bonded phasesprovide excellent stability and selectivity choices with TFA and formic acid mobile phases.The Poroshell 300Extend-C18 column can be used from pH 2-10 for unique separations.These columns can be used for analytical protein separations as well as LC/MS separations.Column SpecificationsBonded Phase Pore Size Temp. Limits* pH Range EndcappedPoroshell 300SB-C18, C8, C3 300Å 90°C 1.0-8.0 NoPoroshell 300Extend 300Å 40°C above pH 860°C below pH 82.0-11.0 YesSpecifications represent typical values only.110 Order online at www.agilent.com/chem/store