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<strong>Column</strong>s for Bioanalytical ChromatographyIs your lab ready for the ever-increasing number –and variety – of bioanalytical <strong>HPLC</strong> applications?Basic peptide separations. High-sensitivity, high-resolution amino acid analyses. Fast-sizeexclusion separations of antibodies. The number of bioanalytical <strong>HPLC</strong> applications continuesto grow at an unprecedented rate. And <strong>Agilent</strong>’s durable – and reproducible – line of ZORBAXcolumns can help you meet your lab’s evolving needs for performance and speed.On the following pages, you will find key facts and specifications about columns forreversed-phase <strong>HPLC</strong>, size exclusion chromatography, ion-exchange chromatography,and hydrophobic interaction chromatography, including:• Poroshell 300 columns – feature a unique, powerful particle for fast, high-resolutionseparations of proteins, polypeptides, and antibodies.• ZORBAX wide-pore 300StableBond columns – deliver long lifetime, high resolution,and good peak shape for peptides and proteins. And they are compatible with commonmobile phases containing TFA.• ZORBAX wide-pore 300Extend columns – ideal for LC/MS of proteins and peptidesat pH 10.• ZORBAX Eclipse Amino Acid Analysis (AAA) <strong>HPLC</strong> columns – a high-efficiency choicefor rapid amino acid separation! These columns make it possible to reduce yourinjection-to-injection analysis time to 14 minutes (7.5 cm column length) and 24minutes (15 cm column length).• ZORBAX GF-250/450 Size Exclusion <strong>HPLC</strong> columns – a rugged, reproducible columnspecially designed for size separations of proteins. Size exclusion columns are compatiblewith organic modifiers and mobile phase denaturants, so you can eliminate proteinaggregation for proper size determination. And they have a separation range of4,000 - 900,000 for globular proteins when using a GF-250 and GF-450 column in series.• <strong>Agilent</strong> Bio-Monolith <strong>HPLC</strong> columns – provide high resolution and rapid separationsof antibodies (IgG, IgM), plasmid DNA, viruses, phages and other macro bio-molecules.Order online at www.agilent.com/chem/store105
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