Agilent HPLC Column Selection Guide

Columns for Special ApplicationsSeparation of Ethiazide (diuretic drug)on ULTRON ES-OVM ColumnColumn: Ultron ES-OVM Chiral7021116514.6 x 150 mm, 5 µmCIH 2 NSOOHNSOHCNHOC 2 H 5Mobile Phase: 20 mM KH 2 PO 4 (pH 4.6)Flow Rate: 1 mL/minTemperature: 25°CDetector: 220 nm1020Time (min)LCUC002Chiral Separation of Warfarin EnantiomersR and S Limit of Quantitation %RSD at 100 fg/mLColumn: Ultron ES-OVM Chiral7021116102.0 x 150 mm, 5 µmTemperature: 30°CInjection 5 µLVolume:Autosampler 10°CTemperature:Needle Wash: Flush port (50:25:25 H 2 O,IPA:MeOH:H 2 O, 5 seconds)Mobile Phase: 83% A = H 2 O + 5mMAmmonium Formate17% B = ACNFlow Rate: 0.5 mL/minStop time: 7.0 minMS Conditions: Agilent 6410A Triple QuadrupoleLC/MS/MS with MultiMode SourceIon Mode: ESI, Negativex10 25.505.255.004.754.504.003.753.503.253.002.752.502.001.751.501.251.000.750.500.250Source ConditionsCapillary Voltage: 2000 VDrying Gas (nitrogen): 5 L/minDrying Gas Temperature: 300°CNebulizer Gas (nitrogen): 40 psiVaporizer 200°CR %RSD = 3.6Product Ion ScanMass Range: 50-500 m/zScan Speed: 500 msecMRM acquisition (Q1 peak width =1.2 and Q2 peak width = 0.70 amu)Delta EMV: 1000VS %RSD = 4.93.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 5.0 5.1Counts vs. Acquisition TimeWarfarinUltron ES Chiral ColumnsDescriptionSize (mm)Particle Size(µm)ES-OVMUSP L57ES-PepsinAnalytical 4.6 x 250 10 724111653Analytical 4.6 x 150 5 702111651 822111651Analytical, with Guard 4.6 x 150 5 702111651A 822111631ANarrow Bore 2.0 x 150 5 702111610Guard Column 4.0 x 10 5 712111630 832111630104 Order online at www.agilent.com/chem/store