Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
<strong>Column</strong>s for Special ApplicationsSuperior Loadability on <strong>Agilent</strong> Prep C18with Basic Compounds<strong>Column</strong>: <strong>Agilent</strong> Prep C18443905-9024.6 x 150 mm, 5 µmMobile Phase: 50% 0.1%TFA:50% ACNFlow Rate: 1 mL/minSample: 10 µLDoxepin/Amitriptyline0.5-50 mg/mL<strong>Agilent</strong> Prep columns show better resolutionand loadability than competitor columns.4000350030002500200015001000500mAU00<strong>Agilent</strong> Prep C18mAUα = 1.64035α = 1.573025201510501 2 3 4 5 6 7 8 90Time (min)Competitor W-C181 2 3 4 5 6 7 8 9Time (min)LCPLC01Steroids: Easy Scalability Using <strong>Agilent</strong> Prep <strong>Column</strong>s<strong>Column</strong> A: <strong>Agilent</strong> Prep C18443905-9024.6 x 150 mm, 5 µm<strong>Column</strong> B: 443905-10221.2 x 150 mm, 5 µm<strong>Column</strong> C: 413910-30230 x 150 mm, 10 µm<strong>Column</strong> D: 413910-50250 x 150 mm, 10 µmMobile Phase: 55% Water:45% ACNFlow Rate: 0.7 mL/min14.87 mL/min29.77 mL/min85.37 mL/minTemperature: AmbientDetector: 240 nmSample: 2 µL42.4 µL170 µL488 µL<strong>Agilent</strong> Prep C18 shows excellent scalability making method transfersimple and predictable.2. Testosterone (in MeOH @ 1mg/mL)A 1 20 2 4 6 8 10 12B10 2 4 6 8 10 12C10 2 4 6 8 10 12D11. Hydrocortisone0 2 4 6 8 10 12Time (min)222LCPLC02Order online at www.agilent.com/chem/store101
Change language