Agilent HPLC Column Selection Guide

Columns for Special ApplicationsAgilent Prep LC Columns• High loadability for maximum sample purification• Easy scalability from 4.6 mm ID up to 50 mm ID for rapid method development• High throughput 21.2 mm ID cartridges for fast purification• Exceptional column stability and loadability up to pH 10Agilent Prep LC columns are designed for high loadability to purify milligram to gramquantities of products. Preparative sized columns are available in 21.2, 30, and 50 mminternal diameters with lengths ranging from 50-250 mm. Columns are available in5 and 10 µm particle sizes with very high efficiency in every dimension. These column choicesaccommodate almost every preparative sample.Agilent Prep 21.2 mm ID columns are available with Agilent’s Preparative Cartridge Hardware.This reliable cartridge hardware makes it simple to use columns with different lengths toincrease sample load. Guard columns are easily integrated onto these columns providingsuperior protection of the analysis column. Analytical sized 4.6 mm ID scalar columns areavailable for method development and optimization prior to scaling up to larger columns.Bulk material is also available.Agilent Prep columns are available in a C18 bonded phase suitable for purification of a widevariety of non-polar and polar compounds. Unbonded silica columns are also available.Column SpecificationsBondedPhasePore SizeSurfaceArea Temp. Limits pH Range EndcappedCarbonLoadC18 100Å 400 m 2 /g 60°C* 2.0-10.0 Single 24%Silica 100Å 400 m 2 /g ** 1.0-8.0 N/A N/ASpecifications represent typical values only.*Temperature limits are 60°C up to pH 8, 40°C from pH 8-10.**Temperature limits for bare silica are determined by the pH of the mobile phase.100 Order online at www.agilent.com/chem/store