Enzyme Action: Testing Catalase Activity - Vernier Software ...

LabQuest 6A• Measure the production of oxygen gas as hydrogen peroxide is destroyed by the enzymecatalase or peroxidase at various pH values.• Measure and compare the initial rates of reaction for the enzyme at each pH value.MATERIALSFigure 1LabQuestenzyme suspensionLabQuest appiceVernier O 2 Gas SensorpH buffers400 mL beaker test tube rack10 mL graduated cylinder thermometerthree 18 × 150 mm test tubesthree dropper pipettes250 mL Nalgene bottle Logger Pro (optional)3.0% H 2 O 2PROCEDURE1. Obtain and wear goggles.2. Connect the O 2 Gas Sensor to LabQuest and choose New from the File menu. If you have anolder sensor that does not auto-ID, manually set up the sensor.3. On the Meter screen, tap Rate. Change the data-collection rate to 0.2 samples/second and thedata-collection length to 180 seconds.6A - 2Biology with Vernier