Standards for Female and Male Sterilization Services - STATE ...

Standards for female and male Sterilization services 33b)Sterilization by Chemical MethodDecontaminated, cleaned, and dried items are put in 2% glutaraldehyde solution forat least 8 to 10 hours.Items such as scissors and forceps should be put into the solution in an openposition.Do not add or remove any items once the timer starts.Items should be rinsed well with sterile water (not boiled water), air-dried, and storedin a covered sterile container for up to 7 days. Sterile water can be prepared byautoclaving water for 20 minutes at 15 lb/sq inch in an autoclave.This method is most suitable for endoscopes and plastic cannulae.3.5.5. Processing LaparoscopesLaparoscopes and accessories should be sterilized or should undergo HLD using the chemicalmethod by soaking in 2% glutaraldehyde solution. All steps of the decontamination andcleaning process must be followed before the laparoscopes and accessories are put in thechemical solution.a)b)c)d)Decontamination: Immediately after use, gently wipe the laparoscope, fibreopticlight source, and cable and plastic tubing with luer lock using a cloth soakedin 60–90% ethyl or isopropyl alcohol to remove all blood and organic material.Cleaning: Place the dissembled parts of the laparoscope in a basin of cleanwater. Wash all outer surfaces using a soft cloth. Clean the inner channels with aclean brush supplied with the laparoscopic kit.High-level disinfection: Put clean and dried dissembled equipment in a basincontaining 2% glutaraldehyde solution for 20 minutes. For the disinfection to beeffective, all parts of the laparoscope must be fully immersed and the disinfectantmust touch all the surfaces of the instrument. Rinse twice with HLD water (waterboiled for 20 minutes and cooled) to remove all traces of the disinfectant.Sterilization: To sterilize, soak the clean and dried dissembled laparoscope in 2%glutaraldehyde solution for 8 to 10 hours. Rinse twice with sterile water to completelyremove all traces of the disinfectant and store in a sterile covered container.