Lipofectamine-Plus® pcDNA Transfection of Vero Cells or A549 Cells
Lipofectamine-Plus® pcDNA Transfection of Vero Cells or A549 Cells
Lipofectamine-Plus® pcDNA Transfection of Vero Cells or A549 Cells
- No tags were found...
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
<strong>Lip<strong>of</strong>ectamine</strong>-Plus ® <strong>pcDNA</strong> <strong>Transfection</strong> <strong>of</strong> <strong>Vero</strong> <strong>Cells</strong> <strong>or</strong> <strong>A549</strong> <strong>Cells</strong>Page 2 <strong>of</strong> 26. Add 2.5 ml <strong>of</strong> DNA-<strong>Lip<strong>of</strong>ectamine</strong> complex/well f<strong>or</strong> 75 cm 2 flasks <strong>or</strong> 5.0ml/150 cm 2 .7. Incubate f<strong>or</strong> 3 hours at 37 o C8. Following the 3 hour incubation, add 5 ml <strong>of</strong> DME-20% (2x FCS) to the 75cm 2 flasks <strong>or</strong> 10 ml to the 150 cm 2 flasks.Day 5: (Monday)Give flask to Les f<strong>or</strong> purification <strong>or</strong> freeze in –70 o C
Change language