1 GENERAL BIOLOGY LAB 1 (BSC1010L) Lab #10: Biotechnology ...

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d. Let the spreader cool for about a minute. Note: If the cell spreader is too hot, youwill kill the E. coli host cells. Once cool, use the spreader to distribute the cellsuspension evenly over the surface of the plate (See Fig. 5) and then replace thelid of the Petri dish.Figure 5.e. Return the cell spreader to the ethanol container without flaming. Repeat steps 16c and d for EACH PLATE.20. Allow the plates to dry for several minutes. Tape the 4 plates together and place themupside down in the incubator for 24-48 hours. Your TA will transfer them to therefrigerator until next week’s lab._____________________________________________________________________________TASK 2 - Recording Results (To be completed during the next lab session)1. Retrieve your group’s plates from the refrigerator.2. Open each plate, one by one, to determine if growth of E. coli occurred. If growth didoccur please note the type of growth - lawn vs. colonial (See Figs. 6A and B).8
A. B.Figure 6. Plates illustrating A. Lawn Growth and B. Colonial growth of E.coli3. The circles below represent each of the four petri dishes tested in this lab. Draw yourgroup’s results for each petri dish. Make sure to label the plates appropriately.9
Page 3 and 4: Table 1:LB -Condition Expected resu
Page 5 and 6: Figure 17. Return the + DNA tube to
Page 7: 17. Using a sterile transfer pipett
Page 11 and 12: c. Since only a portion of the + DN
Page 13: 5. Can you think of a case where th

1 GENERAL BIOLOGY LAB 1 (BSC1010L) Lab #10: Biotechnology ...

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