Nucleic Acids as Therapeutic Agents - icaam

Fig. 11.1 Inhibition of translation ofspecific mRNAs by antisense (AS)nucleic acid molecules.Promoter and polyadenylationregions are p and pa, respectively;the intron is indicated by the letter A;and the exons are indicated bynumbers (1 and 2).(A) An antisense cDNA is clonedinto an expression vector and theconstruct is transfected into a cell,where the antisense RNA issynthesized. Antisense RNAhybridizes to target mRNA, andtranslation is blocked.(B) An antisense oligonucleotide isintroduced into a cell, and after ithybridizes with the target mRNA,translation is blocked.AB