Trypsin / Trypsin-Versene (EDTA) / Versene (EDTA) - Biocenter

Lonza Walkersville, Inc.www.lonza.combiotechserv@lonza.comTech Service: 800-521-0390Document # TS-17-160-2 06/07Walkersville, MD 21793-0127 USA© 2007 Lonza Walkersville, Inc.Trypsin / Trypsin-Versene (EDTA) / Versene (EDTA)Product Use• Trypsin (17-160) is a clear, colorless-yellow,highly active porcine enzyme preparation, usedto remove cells from the culture vessel surfaceinto suspension. It is generally used at a workingconcentration of 0.25%, prepared in a balancedsalt solution, without calcium or magnesium, at apH of 7.6-7.8. After treatment, trypsin activitycan be halted by adding a serum media mixture,or a Trypsin inhibitor (from soybean, forexample) in serum-free systems.• Trypsin-Versene (17-161) or Trypsin-EDTA(ethylenediaminetetraacetic acid) is a combinedreagent used to remove cells from the culturevessel surface into suspension. The EDTA, achelator of divalent cations, is added to trypsinsolutions to enhance activity. The calcium andmagnesium in the extracellular matrix, with aidsin cell-cell adhesion, also obscures the peptidebonds that trypsin acts on. The EDTA is addedto remove the calcium and magnesium from thecell surface which allows trypsin to hydrolyzespecific peptide bonds. This activity can behalted by adding a serum media mixture, or aTrypsin inhibitor (from soybean, for example) inserum-free systems. It is supplied at usedilution.• Versene (17-711) or EDTA (ethylenediaminetetraaceticacid) is a chelating agent used to liftsome cell types from the culture vessel surfaceinto suspension. It can be used as a wash priorto the addition of a trypsin product or used asdescribed above. It is supplied at use dilution.Usage17-160 Dilute 1:10 with HBSS17-161 Use as is17-711 Use as isFormulaComponent(mg/L) 17-160 17-161 17-711Glucose 1,000 1,000 --KCl 400 400 200KH 2 PO 4 60 -- 200NaCl 8,000 8,000 8,000NaHCO 3 -- 580 --NaH 2 PO 4 •7H 2 O 90 -- 2,170Phenol Red -- 2.00 --Trypsin 25,000 500 --Versene (EDTA) -- 200 200SpecificationsSterilityMycoplasma17-160 Negative Negative17-161 Negative Negative17-711 Negative --StoragepH6.44-8.047.10-7.907.08-7.50-10ºC to -20ºC 17-160 and 17-16115ºC to 30ºC 17-711Osmolality(mOsm)Activity/ToxicityEndotoxin(EU/ml)313-565 Pass --269-297 Pass FIO275-291 Pass FIOProduct Use StatementTHESE PRODUCTS ARE FOR RESEARCH USEONLY. Not approved for human or veterinary use,for application to humans or animals, or for use inclinical or in vitro procedures.All trademarks herein are marks of Lonza Groupor its subsidiaries.1

Ordering InformationCatalog DescriptionSizeNumber17-160E Trypsin (10X) 100 ml17-160F Trypsin (10X) 500 ml17-161E Trypsin-Versene (EDTA) (1X) 100 ml17-161F Trypsin-Versene (EDTA) (1X) 500 ml17-711E Versene (EDTA) 0.02% 100 mlTS-17-160-2 06/07All trademarks herein are marks of Lonza Groupor its subsidiaries.2