Nepal Elephant TB Control and Mgt Action Plan.pdf - Elephant Care ...

Nepal Elephant Tuberculosis Control andManagement Action Plan (2011-2015)Check list for fixed ssues: Preserve all ssues in group I and as many ssues in groups II and II as possible.Use 10% buffered formalin at a ra o of ~ 1 part ssue to 10 parts solu on. Cut ssues no thickerthan 0.5 cm. Take 2-3 sets. Submit one set for histopathology and bank 2 sets. Freeze 3-5 cm blocks ofssue from lesions and major organs (lung, liver, kidney, spleen, heart) in small plas c bags or n foil.Freeze post mortem serum (from heart); urine; and any abnormal fluid accumula ons.Group I__ heart (sec ons from auricles, ventricles and valves)__ lung (specimens from several lobes)__ trachea (may be site for TB)__ liver (3 representa ve specimens and bile duct)__ kidney (cortex and medulla of each kidney)__ adrenal gland__ spleen (representa ve cross sec on with capsule)__ lymph nodes (submandibular, bronchial, mesenteric, and tonsillar ssue)__ pancreas__ stomach (several specimens from all areas)__ intes nes (3cm representa ve specimen from each region)__ thyroid gland (and parathyroid if possible)Group II__ urinary ssue (ureters, urethra, bladder cross sec on including mucosa)__ uterus and ovaries (transverse sec ons of cervix, uterine horn, and ovaries)__ testes and epididymus__ prostate, seminal vesicles, bulbo-urethral gland37