HPSEC-ELSD æ³•æµ‹å®šå›ºå ƒèƒ¶å›Šä¸­å¤šç³–çš„åˆ†å­é‡åŠå ¶åˆ†å¸ƒ

2011 1 33 1 Chinese Traditional Patent MedicineJanuary 2011Vol. 33 No. 1HPSEC-ELSD 323000 HPSEC-ELSD HPSEC-ELSD 、。 ShodexOhpak SB-805 /804 /803 0. 4 mL /minELSD 50 ℃ 30 μL。 1. 8 × 10 2 ~ 2. 0 × 10 6 R 2 = 0. 997 2 RSD = 0. 08% n = 5。 HPSEC-RID。 R927. 2 A 1001-1528201101-0079-03Determination of molecular weight and molecular weight distribution of polysaccharidein Guyuan Capsule by HPSEC-ELSDCHEN Qin-mingLIU BinCHEN Wei-pingLishui Municipal Institute for Food and Drug ControlLishui 323000ChinaKEY WORDSHPSEC-ELSDGuyuan Capsulepolysaccharidemolecular weightmolecular weight distributionABSTRACTAIMTo establish a method for determining the molecular weight and molecular weight distributionof polysaccharides in Guyuan Capsuleraw astraglan and Rhizomes of Ginsengby HPSEC-ELSD. METHODSThe Shodex Ohpak SB-805 /804 /803 columns were used in series. The pure water was used as mobile phase. Theflow rate was set at 0. 4 mL /min. The impactor of the ELSD was made open and the temperature of the drift tube inELSD was set at 50 ℃ . The sample size was 30 μL. RESULTSThe standard curve was linear over the range of1. 8 × 10 2 - 2. 0 × 10 6 R 2= 0. 997 2sampling repeatedly for 5 timesRSD = 0. 08% . CONCLUSIONThemethod is steady with perfect reproducibilitybut as compared with HPSEC-RID this result is different. 3 。HPSEC RID1 ELSD RID ELSD HPSEC-ELSD HPSEC-ELSD HPSEC-RID、。。1 1. 1 Agilent1100 Alltech-2000ES 2 High ELSDRID CP225D Performance Size Exclusion ChromatographyHPSEC RE-52AA 、 、TDL-40C 2010-07-221966 - 。Tel05782126848 E-mailcqm60224@ 126. com79

2011 1 33 1 Chinese Traditional Patent MedicineJanuary 2011Vol. 33 No. 1Shodex Ohpak SB-802 / 2. 4 803 /804 GPC 。 2. 4. 1 1. 2 Sigma 50 mg 50 mL 1. 0mg /mL 0. 45 μm 99. 9% 。 20080101、200702022. 4. 2 2. 1 。0 h 5 2 1。2. 1 802 /803 /804 0. 4 mL /min ELSD 50 ℃ 2. 8 mL /min 。1 2. 2 D2000、D1400、D670、D410、D270、D5010 mg 10 mL 1 mg /mL 36 03435 98750. 38750. 49536 013 50. 406 0. 082. 1 36 210 50. 41730 μL 36 049 50. 421GPC Y = -0. 099 6X + 10. 465 R 2 2. 5 2. 3 =2. 4. 1 0. 998 2。2. 1 A、B、C 3 2. 3 1 2. 2 10 g 200 mL 3 500 r /GPC Mn、min 10 min200 mL 30% H 2 O 2 60 mL 50 ℃ D2。 3 h10% 3 500 r /min 6 min50 mL 54 、 3 5 hGYJN I。24 h、36 h 3 50. 448RSD = 0. 07 n = 3、50. 447RSD = 0. 04 n = 3 350. 437 RSD 0. 07% 。 t R /min RSD /%Mw、Z Mz、 Mv1HPSEC-ELSD 200801012HPSEC-ELSD Mn Mw Mz D /%A 1. 32 × 10 6 1. 44 × 10 6 1. 70 × 10 6 1. 11 76. 7320080101n = 5 B 1. 16 × 10 6 1. 16 × 10 6 1. 16 × 10 6 1 9. 75C 8. 74 × 10 5 8. 79 × 10 5 8. 86 × 10 5 1 13. 51A 1. 56 × 10 6 1. 68 × 10 6 1. 80 × 10 6 1. 11 78. 2420070202n = 5 B 1. 05 × 10 6 1. 06 × 10 6 1. 06 × 10 6 1 10. 54C 7. 79 × 10 5 7. 83 × 10 5 7. 87 × 10 5 1 11. 212. 6 RID 1 mg /mL 2. 12. 6. 1 D50、D270、D410、pHD670、D1400 10 mg 10 mL 7. 8 ~ 8. 0RID80

2011 1 33 1 Chinese Traditional Patent MedicineJanuary 2011Vol. 33 No. 1 30 μL GPC 。 ELSD Y = - 0. 140 4X + 12. 673 R 2 = 0. 998 7。2. 6. 2 2. 5 2. 0 mg0. 5 mol /L 。 0. 5 mL 0. 5 mol /L 0. 4 mL /min pH 7. 5 2. 0 mL 。30 μL 3. 3 HPSEC-ELSD HPSEC-RID HPSEC GPC 3 2。 3HPSEC-RID Mn Mw Mz D200801014. 66 × 103 1. 70 × 10 4 4. 89 × 10 3. 7120070202 5. 24 × 10 3 1. 87 × 10 4 5. 02 × 10 4 3. 562HPSEC-RID 20080101、 UV、 、 。3. 2 HPSEC-ELSD 3. 2. 1 3 879-883.HPSEC-ELSD 2009 81142-1144.804 /803 /802 6 2008396955. 。 RID ELSD 6 。《 》2000 RID 2005 2010。ELSD 3 RID 1 2 ELSD RID ELSD RID ELSD 、 7 。。3 HPSEC-ELSD 、3. 1 、 、 、。5 。 H 2 O 2 1YBZ04242005S .2. M . 2008.3. M . 2005114.4 . J . 20062865 . J .. HPLC-ELSD J .7 . J . 2006 24 63. 2. 2 HPSEC 560-565.81