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Organelle-ID RGB III Assay Kitfor detection of the Golgi, ER and Nucleusby microscopyThe endoplasmic reticulum (ER) and Golgi apparatus (GA) are the primary organelles responsible for the proper sorting of lipidsand proteins by cells. Direct membrane continuity between these organelles has been established using different analytical methods,ranging from reconstruction of serial sections in transmission electron microscopy to functional analysis of the transportprocess itself. After synthesis, folding and quality control, the lipid and protein cargo exit from the ER and enter the GA throughthe ER-Golgi interface. Within the ER-Golgi interface, COPII-mediated concentration of membrane and soluble cargo occurs andvarious post-translational modifications take place prior to delivery to the GA, including O-glycosylation, acylation, palmitoylationand mannose-6-phosphate attachment (lysosomal targeting signal). Transient ER-Golgi connections are likely to serve a role inthe diffusion of cargo proteins as well as the recycling of organelle-resident proteins. The structure and functions of the variouscompartments along the secretory pathway are considered complicated and tools for the simple visualization and unambiguouscategorization of the ER and GA in living cells have been lacking.Organelle-ID RGB III Assay KitENZ-51032-K100100 AssaysCompared with other commercially available dyes for labelingGolgi bodies, the green dye component of the Organelle-ID RGB III detection reagent is more faithfully localizedto the Golgi apparatus, with minimal staining of the endoplasmicreticulum. The red dye component of the reagentstains the endoplasmic reticulum with high fidelity and isspecifically designed for use with green fluorescing probes.Organelle-ID RGB III assay kit is validated with humancervical carcinoma cell line HeLa, human T-lymphocyte cellline, Jurkat, canine kidney cell line MDCK, and human boneosteosarcoma epithelial cell line U2OS.The kit should also be suitable for identifying Golgi body andendoplasmic reticulum perturbing agents and thus can bea useful tool for examining the transport and recycling ofmolecules from the GA to ER in cellular secretory pathways.• Assay Golgi and ER dynamics in live cells• Highly resistant to photo-bleaching,concentration quenching and photoconversion• Identify modulators of secretory membranetraffic and organelle integrityFigure 1: Selectivity of organelles.Organelle-ID RGB staining in MDCKcells. The selectivities of the ER-IDRed dye, Golgi-ID Green dye and theHoechst 33342 dye are evident.Figure 2: 3D Imaging of ER (red) andGolgi body (green) displayed relativeto nucleus (blue). Three-dimensionalreconstruction of the spatial relationshipbetween the Golgi body and the endoplasmicreticulum using a structured illuminationmethod. This was accomplished withthe Apotome device from Carl Zeiss, Inc.This imaging hardware enabled creationof optical sections through the cell usinga conventional wide field fluorescencemicroscope, for improved resolution alongthe optical axis. The optical sections werethen used to create a 3-D reconstructionof the endoplasmic reticulum(red) andGolgi body (green), enabling display intheir proper spatial context relative to thenucleus (blue).Product FlyerInternational Edition1-Aug-2010

Ordering InformationProduct Prod. No. SizeOrganelle-ID RGB III assay kitfor microscopyRelated ProductsProduct Prod. No. SizeER-ID Green assay kit ENZ-51025-K500 500 AssaysER-ID Red assay kit (GFP-Certified) ENZ-51026-K500 500 AssaysGolgi-ID Green assay kit ENZ-51028-K100 100 AssaysLyso-ID ® Green detection kit ENZ-51034-K500 500 AssaysLyso-ID ® Red detection kit (GFP-Certified) ENZ-51005-500 500 AssaysMito-ID Red detection kit (GFP-Certified) ENZ-51007-500 500 AssaysMito-ID Green detection kit ENZ-51022-K500 500 AssaysOrganelle-ID RGB reagent I ENZ-53007-C200 200 µlOrganelle-ID RGB reagent II ENZ-53008-C200 200 µlOrganelle-ID RGB reagent IV ENZ-53009-C200 200 µlNucleolar-ID Green detection kit ENZ-51009-500 500 AssaysTotal Nuclear-ID Green/Red nucleolar/nucleardetection kitENZ-51032-K100ENZ-51006-500100 Assays500 AssaysMito-ID Red antifade reagent ENZ-53002-M010 10 mlMito-ID Red antifade reagent with DAPI ENZ-53003-M010 10 mlNorth/South AmericaEnzo Life Sciences International, Inc.5120 Butler PikePlymouth Meeting, PA 19462-1202 / USATel. 1-800-942-0430 / (610) 941-0430Fax (610) 941-9252info-usa@enzolifesciences.comSwitzerland & Rest of EuropeEnzo Life Sciences AGIndustriestrasse 17, PostfachCH-4415 Lausen / SwitzerlandTel. + 41/0 61 926 89 89Fax + 41/0 61 926 89 79info-ch@enzolifesciences.comBeneluxEnzo Life Sciences BVBAMelkerijweg 3BE-2240 Zandhoven / BelgiumTel. +32/0 3 466 04 20Fax +32/0 3 466 04 29info-be@enzolifesciences.comFranceEnzo Life Sciences FRANCEc/o Covalab s.a.s13, avenue Albert Einstein,69100 Villeurbanne, FranceTel. +33/0 472 440 655Fax +33/0 437 484 239info-fr@ enzolifesciences.comGermanyEnzo Life Sciences GmbHMarie-Curie-Strasse 8DE-79539 Lörrach / GermanyTel. +49/0 7621 5500 526Toll Free: 0800 6649518Fax +49/0 7621 5500 527info-de@enzolifesciences.comUK & IrelandEnzo Life Sciences (UK) Ltd.Palatine HouseMatford CourtExeter EX2 8NL / UKTel. 0845 601 1488 (UK customers)Tel. +44/0 1392 825900 (overseas)Fax +44/0 1392 825910info-uk@enzolifesciences.comFor Local Distributors pleasevisit our Website.Enzo, ER-ID, Lyso-ID, Nuclear-ID, Mito-ID, Nucleolar-ID, and Organelle-ID are trademarks of Enzo Life Sciences, Inc. Several of Enzo’s products andproduct applications are covered by US and foreign patents and patents pendingwww.enzolifesciences.comZZ-F0810-1039