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78Michał Szpinda, Marcin Daroszewski11. Roman M.J., Devereux R.B., Kramer-Fox R.,O’Loughlin J.: Two-dimensional echocardiographic aorticroot dimensions in normal children and adults. Am. J.Cardiol. 1989; 64: 507-12.12. Hirata K.: A metrical study of the aorta and main aorticbranches in the human fetus. Nippon Ika Daigaku Zasshi1989; 56: 584-91.Corresponding author:Michał Szpinda MDChair and Department of Normal Anatomythe Ludwik Rydygier Collegium MedicumKarłowicza 24 StreetBydgoszcz 85-092Polandtel. + 48 (052 5853705)fax + 48 (052 5853753)e-mail kizanat@cm.umk.plReceived: 25.11.2008Accepted for publication: 16.12.2008
Medical and Biological Sciences, 2008, 22/4, 79-84ORIGINAL ARTICLE / PRACA ORYGINALNAJustyna Szymańska 1. Małgorzata Łukowicz 1. Krzysztof Góralczyk 2. Magdalena Weber-Zimmermann 1. Danuta Rość 2EFFECT OF LOW LEVEL LASER THERAPY AND HIGH INTENSITY LASER THERAPYON ENDOTHELIAL CELL PROLIFERATION IN VITRO- PRELIMINARY COMMUNICATION1 Chair and Department of Lasertherapy and Physiotherapy, Nicolaus Copernicus University Collegium Medicum in BydgoszczHead: Małgorzata Łukowicz, MD2 Chair and Department of Pathophysiology, Nicolaus Copernicus University Collegium Medicum in BydgoszczHead: Danuta Rość, MD, ass. professorSummaryB a c k g r o u n d . A lot of investigators tried to assumean influence of Low Level Laser Therapy (LLLT) on endothelialcells proliferation in vitro. Endothelial cell (EC) playsa key role in the process of tissue repair. The main purpose ofthis work was to evaluate the influence of LLLT 660 nm,670 nm, 820 nm and HILT 808 nm on the proliferation ofEC.M a t e r i a l a n d m e t h o d s . The tests were done onhuman umbilical vein endothelial cells (HUVEC). Cultureswere exposed to laser irradiation 660 nm and 670 nm atdifferent dosages, power output was 10 – 40 mW, 820 nmwith power 100 mW, 808 nm with power 1500 nm. Energydensity was from 0.28 to 11.43 J/cm 2 . Cells proliferation ofcontrol and tested culture was evaluated with colorimetricdevice to detect alive cells. The tests were repeated 8 times.R e s u l t s . We observed good effects of LLLT on aliveisolated EC and no effects in experiments on previouslydeep-frozen cultures. Also HILT stimulated the proliferationof HUVEC.C o n c l u s i o n . Endothelial cells play a key role invascular homeostasis in a human. We observed the stimulatoryeffect of LLLT and HILT on the proliferation ofHUVEC.A lot of factors influence the proliferation of EC so it isnecessary to continue the experiment with different doses,intensity, cell concentration.Key words: low level laser therapy (LLLT), high intensity laser therapy (HILT), vascular endothelial cells (EC), HUVECINTRODUCTIONLaser is a device able to generate electromagneticradiation characterized by extraordinary properties.Attempts to explain its mechanisms were made during40 years of its application in medicine. It is said thatthe basics are formed at the cellular and molecularlevel [1.2.3]. Endothelium plays a significant role invascular homeostasis maintenance in the human organism.Growth factors produced by the endothelial cellsare responsible for tissues and healing processes aswell as neoangiogenesis [4-7]. Favourbale conditionswithin the blood vessels lead to the rheological bloodproperties, increase blood flow velocity and decreasethe risk of thrombotic embolism.The absorption and diffusion processes that takeplace between blood vessels and interstitial tissue areimproved at the same time, which reduces tissue ischaemia,stimulates metabolism and thus activatesregeneration processes [8-11].MATERIAL AND METHODSThe experiments were carried out basing on thevascular endothelial cells of HUVEC line. The endothelialcells were placed on 96-dot plater characterizedby the hole surface of 0.35 cm 2 . To evaluate the influenceof the various factors which determine proliferationin the experiments, the number of cells within theculture was altered, the parameters of radiation werechanged, living as well as frozen cells were used, sin-
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