medical and biological sciences - Collegium Medicum - Uniwersytet ...

52Jan Styczyński, Anna JaworskaRESULTS AND DISCUSSIONCell lines. After 72 hours of incubation with prednisolonein B-lineage cell lines, the expression ofCD19 decreased, in Raji cell line from MFI=12,99 to10,89 ie. by 16%, and in Daudi cell line fromMFI=7,82 to 5,88 ie. by 25%; while expression ofCD10 increased in the same period, in Raji from 1,80to 2,47, and in Daudi from 2,66 to 3,86. Since thenumber of cells with CD10 expression has decreased(by 5% and 3%, respectively), the upregulation of theexpression of CD10 antigen was dependent on theincrease of mean number of CD10 molecules on cellsurface (from 6151 to 8441 in Raji, and from 9090 to13191 in Daudi, respectively). On the other hand, expressionof dominating immunophenotype in T-lineageCCRF-CEM and Jurkat cell lines decreased during invitro therapy with prednisolone (Fig. 2).Changes in immunophenotype in patient samples.After 3-day prednisolone therapy, expression of specificleukemic markers was decreased. The only exceptionwas an increase of CD3 expression in T-ALL,however a large variability of expression of this antigenwas observed among patients with leukemia.CD10 expression measured by MFI in common-ALLwas decreased 2-fold (p