medical and biological sciences - Collegium Medicum - Uniwersytet ...

Medical and Biological Sciences, 2008, 22/4, 49-53ORIGINAL ARTICLE / PRACA ORYGINALNAJan Styczyński 1 , Anna Jaworska 2QUANTITATIVE ANALYSIS OF CHANGES IN EXPRESSION OF LEUKEMIC MARKERSDURING SHORT-TERM PREDNISOLONE THERAPY IN VITROILOŚCIOWA ANALIZA ZMIAN EKSPRESJI ANTYGENÓW BIAŁACZKOWYCHPODCZAS KRÓTKOTRWAŁEJ TERAPII PREDNIZOLONEM IN VITRO1 Chair and Clinic of Pediatric Hematology and Oncology, Nicolaus Copernicus University in Toruń,Collegium Medicum in BydgoszczHead: Mariusz Wysocki, MD, PhD, professor of medicine2 Students’ Scientific Society, Nicolaus Copernicus University Collegium Medicum in BydgoszczSummaryI n t r o d u c t i o n . Response to treatment in childhoodacute lymphoblastic leukemia (ALL) depends on numerousvariables, including the clinicobiologic features of the disease,chemotherapy regimens and interactions, and the abilityof individual patients to metabolize antileukemic drugs.The objective of the study. The analysis ofin vitro changes in expression of leukemic markers in leukemic/lymphoidcell lines and in children with ALL after shottermincubation with prednisolone.Patients and methods. Sixty children withALL and four leukemic/lymphoid cell lines were tested forimmunophenotype changes occurring after three-day incubationwith prednisolone. Immunophenotype analysis wasperformed with the use of flow cytometry.R e s u l t s . After 72 hours of incubation with prednisolone,the expression of CD19 decreased in Raji and Daudicell lines, while CD10 expression increased in both cell lines.The expression of dominating immunophenotype inT-lineage CCRF-CEM and Jurkat cell lines decreased duringthe culture. In patient samples, the expression of specificleukemic markers decreased after 3-day prednisolone therapyin B-lineage samples. In common-ALL, CD10 and CD19expression decreased 2-fold (p