P. s - Cost 873

Fruit production overviewSpeciesPlumSour cherryPeachScientific namePrunusdomesticaNo. oftrees(million)Yield(t)Usage50 475.000 Brandy, jam,preserve, compote,dried and freshP. cerasus 10 100.000 Frozen, processingP. persica 5 50.800 Fresh, processingSweetcherryApricotWalnutP. avium 2 30.000 Fresh, processingP. armeniaca 1,6 22.000 Fresh, processingJuglans 1,7 17.000 Fresh

pvs. syringae, morsprunorum and persicae• LOPAT group Ia (Lelliottet al., 1966)

Pathogenicity test0101 - P. s. pv. morsprunorum0102 - P. s. pv. persicae0103 - P. s. pv. syringae18 - P. s. pv. syringaeK, W - negative control

PsmFluorescence on King’smedium BPspPssPspPss

Levan production(NSA medium)PsmPspPss

Vitality on NSA medium(Garrett, C.M.E., Panagopoulos, C.G., Crosse, J.E. 1966. Comparison of plant pathogenicpseudomonads from fruit trees. J. appl. Bact.,29 : 342‐356.)Strains 2 days 3 days 4 days 7 daysCatalase NAS Catalase NAS Catalase NAS Catalase NAStesttesttesttestP. s. pv. morsprunorum + + + + - - - -P. s .pv. persicae + + + + + + + +P. s. pv. syringae + + + + + + + +Catalase test (Klementet al., 1990)

Aesculin hydrolysis(Lelliott & Stead, 1987)Ice nucleation(Lindow, 1990; Schaad et al., 2001)PsmPssPositive reaction:dark brown discoloration of the medium0101 - P. s. pv. morsprunorum0102 - P. s. pv. persicae0103 - Ps P. s. pv. syringae018 - P. s. pv. syringaeNez - negative control

Growth in Sucrose NutrientBroth (SNB) Medium(Jones, 1971)Tyrosinase Activity(Jones, 1971)PsmPsmPssPssThe coloration of the broth(white or yellow) isobserved within 1 weekPositive reaction: production of a reddish‐brown discoloration of the medium

Production of syringomicinPCR(Bultreys& Gheysen, 1999)BioassaySyrDgene1 - P. s. pv. syringae2 - P. s. pv. persicae3 - P. s. pv. morsprunorumw – negative controlTest organism G. candidum

Production of coronatine(Bereswill et al., 1994)cfl gene650 bp1 - P. s. pv. morsprunorum2 - P. s. pv. persicae3 - P. s. pv. syringae4 - P. glycineaw – negative control

Rep‐PCR(Schaadet al., 2001)1 - P. s. pv. morsprunorum2 - P. s. pv. persicae3 - P. s. pv. syringaew – negative control