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4 th International Symposium of Biotechnology10 – 11 th November 2011, Bucharest, RomaniaP.III.14. PHYSICAL-CHEMICAL PROPERTIES OF MONASCUSMETABOLITESNicoleta Radu 1 , Yiannis Kourkoutas 2 , Mariana Ferdes 31 USAMV Cluj Napoca and Politehnica University of Bucharest, Bioengineering and BiotechnologyDepartment; e-mail: nicolbiotec@yahoo.com2 Dr. Yiannis Kourkoutas Department of Molecular Biology & Genetics Democritus University ofThrace, Alexandroupolis Greece3 Politehnica University of Bucharest, Bioengineering and Biotechnology DepartmentKeywords: biopigment, food safety, mycotoxin contentMonascal metabolites are widely using as food colorant in Asia, Europe and United States.Bioprocesses, based on solid state or submerged biosynthesis [1-3] are recommended as natural fooddyes, but the safety product is insufficiently solved, due to content of citrinin. Citrinin is a mycotoxinespecially toxic to liver and kidney tissue and is suspected of being a renal carcinogen leading torenal tumors. Used two strains we obtained the biopigments which are characterized by UV-VIS,infrared spectra, mass spectrometry and HPLC, in order to establish the major compounds inMonascus metabolite and the mycotoxin concentration in them. The main conclusion of our study isthe following: metabolites obtaining in solid state or in submerged biosynthesis were a content ofmycotoxin less than 25 ppm and is suitable to use as raw material in food industry.References1.You-Zhi Wang Xiu-Lian Ju and Yu-Guang Zhou (2005), The variability of citrinin production in Monascus typecultures Food Microbiology, (22)145-148.2.Betina, V. (Ed.) (1984), Mycotoxins - production, isolation, separation and purification. Amsterdam: Elsevier.pp. 217-236.3.Blanc, P. J.; Loret, M. O. and Goma, G. (1995), Production of citrinin by various species of Monascus Biotech.Lett., 17 : (3), 291-294.AcknowledgementsThe work was financially supported by the project POSDRU/89/1.5/S/52432 from 1.04.2010 - Institutionalorganization of a postdoctoral school of national interest "Applied biotechnology with impact in the Romanianeconomy"; the project was cofunded by the EU Social Fund in the framework of the Sectorial OperationalProgramme 2007-2013 for Human Resources Development.62
4 th International Symposium of Biotechnology10 – 11 th November 2011, Bucharest, RomaniaP.III.15. HEAT-INDUCED STRUCTURAL CHANGES OF BOVINEΑ-LACTALBUMIN AS MEASURED BY FLUORESCENCESPECTROSCOPYN. Stănciuc, S. Dima, G. Râpeanu, G. BahrimDunarea de Jos University of Galati, 111, Domneasca Street, Building E, Room 304, Galati,Romania, E-mail: nsava@ugal.roKeywords: alpha-lactalbumin, heat treatments, structural changes, fluorescence spectroscopy.α-Lactalbumin (α-La) is a monomeric globular protein with a molecular mass of 14,200and is one of the best understood proteins in protein folding studies. α-La has two domains, an α-domain comprising the residues 1-39 and 81-123 and a β-domain comprising the residues 40-80. Thestructure is stabilized by four disulfide bridges (6-120, 61-77, 73-91, and 28-111). It has calcium(Ca2+)-binding site, for disulfide bond formation and for folding in the reduced denatured protein,calcium ion plays a major role. The two domains are held together by the cystine bridge betweenresidues 73 and 91, forming a loop.α-La serves as a useful model for the protein folding problem since it has several partiallyfolded intermediate states and is known to be present in molten globule form under variousconditions.In this study, the pH and heat-induced conformational changes were analyzed using thefluorescence techniques. The experimental results demonstrate an all-or-none transition at 7.0. Theheat treatment caused the decrease of intrinsic fluorescence and, respectively, the increase in ANSfluorescence intensity.This work was financially supported by the POSDRU/89/1.5/S/52432/2010 Project ”Postdoctoral schools ofnational interest Applied Biotechnology with impact in Romanian bio-economy, acronym PDS-BIOTECH cofinancedby European Social Fund through Operational Programme Human Resources Development 2007- 2013,Priority axis no. 1 Education and professional training to support the growth and development of knowledge-basedsociety, Area of intervention 1.5 Doctoral and postdoctoral programs to assistance the research.BIOALIMENT Research Platform is acknowledged for providing technical support.63
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