biotechnology - SimpBTH - Universitatea De Stiinte Agronomice Si ...
biotechnology - SimpBTH - Universitatea De Stiinte Agronomice Si ...
biotechnology - SimpBTH - Universitatea De Stiinte Agronomice Si ...
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4 th International Symposium of Biotechnology10 – 11 th November 2011, Bucharest, RomaniaP.I.17. RHODIOLA ROSEA L. IN VITRO PLANTSMORPHOPHYSIOLOGICAL AND CYTOLOGICALCHARACTERISTICSKrasimira TASHEVA and Georgina KOSTURKOVAInstitute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaPhone: + 359 2 974 6228; Fax: + 359 2 978 5516Correspondence to: Krasimira Tasheva, E-mail: krasitasheva@yahoo.comKeywords: medicinal plant, golden root, in vitro, cytological and morphological characteristicsRhodiola rosea L. (Golden Root, Roseroot) is an endangered species and is protected bylaw in Bulgaria and other countries. Rhizome and roots extracts are used as a stimulant of immunesystem, adaptogen, and for prophylactics and cure of socially important diseases – cancer,cardiovascular, etc.Schemes for in vitro propagation and plant regeneration were established previously. Theaim of the present work is to characterize morphologically and cytologically regenerants. Concerningflowers, leaves, stem and rhysomes no differences were recorded between the wild plants and theregenerated plants grown ex vitro in the adaptation room, in the green house and in the mountain. Todetect chromosome number, commonly used techniques for squash preparations were modified foruse of Rhodiola rosea root tip meristem cells from regenerants. In vitro plants obtained on differentculture media were subjected to cytological analysis. The chromosome number in all samples was 2n= 22, which confirms the diploid level of plant regenerants and cytogenetic identity with the wildtype.The study indicates that the established in vitro schemes are suitable for mass propagationand production of identical plants of this endangered medicinal species which could be a basis forrestoration of the natural habitats and for establishment of fields for growing plants for production ofpharmacologically valuable substances.46