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4 th International Symposium of Biotechnology10 – 11 th November 2011, Bucharest, RomaniaP.I.13. EFFECT OF SILVER NITRATE ON IN VITRO ROOTFORMATION OF GENTIANA LUTEAMariya PETROVA 1 , Ely ZAYOVA 1 and Antonina VITKOVA 21 Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences2 Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of SciencesCorrespondence to: Mariya Petrova, E-mail: marry_petrova@yahoo.com, Institute of PlantPhysiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, Sofia1113, BulgariaKeywords: Gentiana lutea, silver nitrate, bacterial contamination, in vitro rootingGentiana lutea (Gentianaceae) is valuable, protected species and is included in the RedBook of Bulgaria. Microbial contamination is one of the most critical and often encounteredproblems in plant tissue cultures. The influence of silver nitrate (AgNO 3 ) on in vitro rooting of G.lutea was examined. Considerable limitation of late bacterial contamination and improved in vitrorooting of plants were achieved on ½ MS2 rooting medium supplemented with IBA (1 mg/l) andAgNO 3 (1 mg/l). The optimization of this medium was provided a high degree of rooting (90%) andlack of bacterial contamination. Different concentrations of silver nitrate had the greatest effect onthe various growth parameters. A maximum plant height (2.5 cm), number of roots/plant (4.5) withmean length 1.5 cm was obtained on ½ MS2 after four weeks of culture. The effect of different mixturesubstrate during acclimatization stage was also studied. Best response (65%) was obtained in peat:perlite: sand (2:1:1 v/v/v) after three week of transplantation in ex vitro conditions. This techniquecan be effectively used to in vitro rooting of G. lutea.42