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4 th International Symposium of Biotechnology10 – 11 th November 2011, Bucharest, RomaniaP.I.10. AN ANALYSIS OF THE EFFECT OF TEMPERATURE ONTHE QUANTITY OF BT TOXIN IN COTTON VARIETIESMUHAMMAD SAJJAD ALIi 1 , INTEZAR ALI 21. Universitatea de Stiinte Agronomice si Medicina Veterinara, Bucuresti, Romania2. University of Agriculture, Faisalabad, PakistanKeywords: Cry toxin; quantity; temperature; correlationThe expression of Bt transgene in cotton cultivars is dependent upon a host of factors. Extremevariations in temperature not only affect overall plant vigour and growth but the expression ofCry1Ac gene is also compromised. The expression of Bt content correlated with temperature wasquantified in six cotton varieties during the cotton year 2010-2011. The results indicate a decline inexpression of Cry1Ac over temperature independent of the hybrid genotype. The situation necessitatesa post commercialization monitoring of the formally approved cotton varieties.P.I.11. A MODEL POST COMMERCIALIZATION MONITORINGFRAMEWORK FOR BT COTTON IN PAKISTANMUHAMMAD SAJJAD ALIi 1 , INTEZAR ALI 21. Universitatea de Stiinte Agronomice si Medicina Veterinara, Bucuresti, Romania2. University of Agriculture, Faisalabad, PakistanKeywords: Bt cotton; stakeholders; monitoring; frameworkCotton varieties were formally approved for large scale cultivation in Pakistan in late 2010.However,the prevalence of Bt cotton varieties in farmers’ fields can be traced back since 2002.The cultivationof unapproved varieties with an unknown parent germplasm and a doubtful compatibility of suchvarieties with local flora must be analysed for environmental consequences. The flow of transgene inlocal ecosystem needs to be monitored at earliest. A regulatory model is designed for the monitoringof ill effects/spread of Bt transgene. The model presents a plan from sampling to results interpretationinvolving the active role of all agricultural stake holders in Pakistan.40