biotechnology - SimpBTH - Universitatea De Stiinte Agronomice Si ...

4 th International Symposium of Biotechnology10 – 11 th November 2011, Bucharest, RomaniaO.III.6. DETERMINATION OF CHEESE ORIGIN BY USING26S RDNA FINGERPRINTING OF YEAST COMMUNITIES BYPCR DGGE: AN APPLICATION ON FRIED CHEESE OFWIELKOPOLSKA REGIONTomasz RYCHLIK, Edna ARCURI*, Didier MONTET*, Jacek NOWAK andZbigniew CZARNECKIPoznan University of Life Sciences, Institute of Food Technology of Plant Origin, Department ofFermentation and Biosynthesis, 31 Wojska Polskiego street, 60-624 Poznań, Polantel. 48618487278, fax 488487314, tomrych@up.poznan.pl*Cirad, UMR 95 Qualisud TA B-95/16, 73 Jean-François Breton street, 34398 Montpellier, France,tel. 33467615728, fax 33467614433, didier.montet@cirad.frProtected Geographical Status is a system which protects the authenticity of traditionaland/or regional food products in the European Union. Fried Cheese from the Wielkopolska region(west part of Poland) is one of the few traditional products labeled as Protected GeographicalDesignation in Poland and should not be manufactured in any other part of the country. To eliminateunfair competition and the misleading of consumers by promoting non-genuine products, it isnecessary to create an effective traceability system of food articles. The main aim of this study was topropose an analytical tool that will permit to link microbial ecology to geographical origin of thefood. Polymerase Chain Reaction – Denaturing Gel Gradient Electrophoresis (PCR-DGGE) system amolecular tool that permits to analyze in a single step all the yeasts present in the cheese matrix withthe objective to link yeast communities to the geographical origin and avert the single evaluation ofeach yeast strain by sequencing. For this purpose, molecular techniques employing 26S rDNAprofiles generated by PCR-DGGE were used to assess the variation in the yeast community oftraditional fried cheeses from six different producers, five from the Wielkopolska and one fromSilesia. Statistical analysis of PCR-DGGE profiles obtained after direct DNA extraction andamplification showed significant differences in migration patterns on the DGGE gel. Some commonbands appeared in all of the samples, regardless of location and producer. The band profiles ofcheeses from different sources were specific for almost each district and in particularly the productfrom Silesia which is not protected by UE law. This method seems to be an effective traceability toolproviding biological bar code which allows tracking back the food to their authentic location.22