ACE Excel Ultra Robust UHPLC Columns - Winlab.com.au

ExcelTMUltra Robust UHPLC Columns• High efficiency 2µm particles• Unique phases for rapid method development• Compatible with UHPLC and HPLC systems• HSC TM High Stability Columns – ultra robust columns up to 1,000 bar (15,000psi)UHPLC ColumnsExcelTM

ACE ® Excel TMUHPLC ColumnsDesigned to take full advantage of low dispersion, ultra-highpressure UPLC ® and UHPLC instruments, the introduction ofACE ® Excel TMUHPLC columns gives chromatographers morechoices to achieve better results.Ultra robust columns – Unique phases – High efficiency 2µm particlesNEW High Efficiency 2µm ACE ParticlesACE Excel UHPLC columns are available in a new high efficiency 2µm particle size. Selectivity is unchanged fromexisting 3µm, 5µm and 10µm ACE HPLC phases. Excellent peak shape, great column-to-column reproducibilityand exceptional column ruggedness is now available to UPLC and UHPLC users with ACE Excel UHPLC columns.HSC TM High Stability ColumnsAll ACE Excel UHPLC columns are manufactured using a proprietary HSC (High Stability Column) manufacturingprocess that results in ultra robust UHPLC columns.Optimised UHPLC HardwareACE Excel columns utilise specially developed low dispersion hardware which enables high efficiency UHPLCseparations up to a maximum pressure limit of 1,000 bar (15,000psi).Unique Stationary PhasesACE Excel 2µm columns are available with C18-PFP and C18-AR “ExtraResolving Power” phases. These two unique phases offer multiple mechanisms ofseparation and have been specifically developed to provide alternative selectivityto C18 phases. ACE Excel 2µm columns are also available with C18, C8, C4, CN,AQ, Phenyl and Silica phases.ACE ® StationaryPhases VirtuallyEliminate theNegative Effectsof Silanols on UHPLCand HPLC SeparationsSi-OHBetter UHPLC ResultsThe introduction of ACE Excel UHPLC columns means chromatographers now have more choices within theirreach to achieve better results with their UPLC and UHPLC instruments.Performance Boost for HPLC InstrumentationACE Excel 2µm UHPLC columns provide significantly lower back pressure than other UPLC and UHPLC columnspacked with

ACE ® Excel TM Ultra Robust UHPLC ColumnsHigh speed, high resolution, UHPLC separationsACE ® Excel TM 2µm UHPLC columns• Fully compatible with all commercial UPLC and UHPLC instruments• Brings renowned ACE HPLC columns’ advantages to UHPLC columns• Delivers a high level of reliability and ruggedness• Offers easy scalability from UHPLC to HPLC to preparative HPLC• Provides users of UPLC/UHPLC with additional choices in stationary phases, includingthe powerful C18-AR and C18-PFP phasesFigure 1: ACE ® Excel TMunique stationary phase columns provide alternative selectivity with high speed, highresolution separationsApplication #1500 – HPLC to UHPLC OptimisationSample: 1) 1,2-dimethoxybenzene 2) 1,3-dimethoxybenzene 3) 1,3,5-trimethoxybenzene 4) tolueneMobile Phase: MeOH/H 2O (50:50 v/v) Temperature: 40°C Detection: UV, 210 nm3HPLC(~13 minutes)R s= 1.71 2Waters XBridge 5 C18150 x 4.6 mm, 1.00 ml/mint r4= 13.60 mins43UPLC(~4.5 minutes)R s= 1.1t r4= 4.69 mins4Waters ACQUITY 1.7 BEH C1850 x 2.1 mm, 0.21 ml/min1 234UHPLC(

ACE ® Excel TM Ultra Robust UHPLC ColumnsAll the well known advantages of ACE ® HPLCcolumns are now available for UHPLCACE ® Excel TM Advantages• Excellent peak shape for basic andacidic compounds• Exceptional column-to-columnreproducibility• Rugged and reliable day-to-dayperformance and superb columnlifetimeFigure 2: ACE ® Excel TM delivers excellent resolution and peak shapeApplication #1501 – Pharmaceuticals and Related Compounds by UHPLCMobile Phase: A = 5 mM formic acid in H 2Oand B = 5 mM formic acid in MeOHGradient: 3 to 100% B in 5 minutesFlow Rate: 0.6 ml/minTemperature: 40°CDetection: UV, 254 nmColumn Dimensions: 50 x 2.1mm1. paracetamol2. hydrochlorothiazide3. methylphenylsulphoxide4. methylphenylsulphone5. aspirin6. phenacetin7. 1,3-dinitrobenzene8. 1,2,4-trimethoxybenzene9. ethylbenzoate10. nimesulide11. ibuprofen12. indomethacin13. mefenamic acidLeverage the power of bonded phaseselectivity and combine it with UHPLCefficiency and speedACE Excel 2 C182 13, 45768109P max= 374 bar121311C18 bonded phases are the mostpopular phases for HPLC/UHPLCbecause they offer good retention andselectivity for a wide variety of sampletypes. In addition, they are perceived tobe more rugged and reliable than otherbonded phases. C18 bonded phasesdepend mainly on hydrophobicinteractions (and occasionally shapeselectivity) to achieve satisfactoryseparations. Although versatile, C18phases offer a limited number ofseparation mechanisms and this maylead to less than optimum separationsin some cases and complete lack ofresolution of important peak pairsin others.Zorbax Eclipse1.8 XDB C18Waters ACQUITY1.7 BEH C18PhenomenexKinetex 1.7 C182 13, 42 1341, 234 575576676888101091099P max= 540 bar121311P max= 581 bar121311P max= 540 bar121311ACE columns have led the way inoffering C18 bonded phases with theadvantage of ‘extra selectivity’.C18-AR and C18-PFP bonded phaseshave proved to be extremelypowerful tools to use in leveraging this‘extra selectivity’ to achieve separationsthat may not be possible with‘standard’ C18 bonded phases. These‘extra selectivity’ phases are not meantto replace ‘standard’ C18 bonded phases,but rather complement them and soprovide chromatographers withpowerful, additional mechanisms ofseparation that can be used to achievebetter overall chromatographic results.The introduction of ACE Excel UHPLCcolumns means that chromatographersnow have more choices within theirreach to achieve better results withtheir UPLC and UHPLC instruments.ACE Excel 2 C18-ARACE Excel 2 C18-PFP121 234 50 1 2 3 45MinutesComparative data may not be representative of all applications.Please see p.10 for acknowledgement of trademarks.34 5The above example highlights that these leading C18 brands providesimilar selectivity and fail to fully resolve the components under theseconditions, with 2 or more critical pairs non baseline resolved in each case.The unique selectivity of the ACE Excel C18-AR and the ACE Excel C18-PFPphases enable improved separations.6687789P max= 364 bar12101311P max= 365 bar1210139 112ACE Excel Ultra Robust UHPLC Columnswww.ace-hplc.com

ACE ® Excel TM Ultra Robust UHPLC ColumnsFully compatible with all commercial UPLC andUHPLC instrumentsCompatible with all UPLC and UHPLC SystemsACE Excel UHPLC columns are designed to be fullycompatible with all commercial UPLC and UHPLCinstruments and have been engineered to benefitfrom the high flow rate, ultra-high pressure and lowdispersion of these systems. ACE Excel columns providechromatographers with more column choices sothat even more value can be obtained from theirUPLC/UHPLC instruments.Performance Boost for HPLC SystemsOwing to the optimal 2µm particle size and a rigorousclassification protocol, column back pressure issignificantly lower than for traditional UPLC and UHPLCcolumns packed with

ACE ® Excel TM Ultra Robust UHPLC ColumnsColumn-to-column reproducibility and ultrarobust, reliable performanceExcellent ReproducibilityColumn-to-column reproducibilityis affected by a number of factorsincluding the production of the silicastationary phase support, themanufacture of the silane stationaryphase, the bonding of the stationaryphase to the support and thepacking of the stationary phase intothe column. The more tightly eachstage of the column manufacturingprocess is controlled, the better thequality of the resultant column will be.ACE columns are subjected to extensivecontrols at each stage of themanufacturing process, to ensurethat ACE columns produce reliable,predictable high performanceseparations from column-to-columnand batch-to-batch.Figure 4: ACE ® Excel TM UHPLC columns are exceptionally durableApplication #1506% Initial Efficiency120100806040200Prototype 2µm C18 Column(column manufactured withoutHSC packing technology)Number of InjectionsACE Excel 2 C18 UHPLC Column(column manufactured with HSCpacking technology)0 500 1000 1500 2000Fast Gradient Conditions:Column Dimensions: 100 x 2.1 mm Flow Rate: 0.73 ml/min P max= 1,000 bar (15,000psi)Mobile Phase: A = 0.1% formic acid in H 2O B = 0.1% formic acid in MeOHGradient: 20% to 90%B in 6 minutes, 1 minute hold90% to 20%B in 1 minute, 2 minutes re-equilibration holdHSC TM High Stability ColumnsAll ACE Excel UHPLC columns aremanufactured using a proprietaryHSC (High Stability Column) packingtechnology, which results in ultra robustcolumns.ACE Excel 2 C18 – StartNot only are ACE Excel columns packedexceptionally well, they incorporate aninlet frit design that reduces the risk ofplugging.0 1 2 3 4 5 6 7MinutesIt is still recommended that you filtersamples and mobile phases as youwould with any UHPLC column, butACE Excel columns provide the samelevels of durability and lifetime that youwould expect from HPLC columns.ACE Excel 2 C18 – After 2,000 fast gradient cycles0 1 2 3 4 5 6 7MinutesEfficiency and peakshape unchangedFigure 4 illustrates the excellent columnlifetime provided by an ACE Excelcolumn under searching fast gradientUHPLC conditions.A 100 x 2.1mm ACE Excel 2µm C18 UHPLC column manufacturedusing the proprietary HSC (High Stability Column) packingtechnology was subjected to a gradient cycle incorporating a P maxof1,000 bar (15,000psi). After over 2000 cycles, the column efficiency,peak shape and retention times were essentially unchanged.In contrast a Prototype 2µm C18 column manufactured from thesame silica but without the proprietary HSC column packingtechnology was seen to rapidly deteriorate.4ACE Excel Ultra Robust UHPLC Columnswww.ace-hplc.com

ACE ® Excel TM Ultra Robust UHPLC ColumnsEasy scalability from UHPLC to HPLC toPreparative HPLCFigure 5: Selectivity is preserved and assured when scaling ACE ®stationary phases from UHPLC (2µm) to HPLC (3µm and 5µm) topreparative HPLC (10µm)Application #1502 – Reproducible Scale-upMobile Phase: 35:65 MeCN/0.1%TFA in H 20Temperature: 22°CWavelength: 254 nmACE Excel 2 C18150 x 2.1mm(0.21ml/min)1231) uracil2) 4-hydroxybenzoic acid3) acetylsalicylic acid4564) benzoic acid5) 2-hydroxybenzoic acid6) ethyl parabenUHPLCAll ACE Excel UHPLC columns areavailable in larger particle sizeequivalents and larger non-analyticaldimensions.For ACE stationary phases, thesame controls that ensure highlyreproducible columns from batch-tobatchalso ensure that you can transfermethods more easily from UHPLCto HPLC, or vice-versa, or scale-upto preparative applications whenneeded to isolate or purify a targetcompound. The column efficiencywill, of course, change, but theselectivity will be predictably the same.ACE 3 C18150 x 3.0mm(0.40ml/min)123456ScalableFullyHPLCACE 5 C18150 x 4.6mm(1.00ml/min)123456ScalableFullyACE 10 C18150 x 21.2mm(21.2ml/min)1 230 1 2 3 4 5 6 7Minutes456PrepHPLCThese chromatograms of a test sample run under the same mobilephase conditions on an ACE Excel 2µm C18 UHPLC column, an ACE3µm C18 HPLC column, an ACE 5µm C18 HPLC column and an ACE10µm C18 preparative HPLC column illustrate the consistentselectivity inherent in all ACE stationary phases, regardless of particlesize. This permits easier transfer of methods from UHPLC to HPLCor HPLC to UHPLC. It also makes it easier to scale-up from analyticalto preparative applications.UHPLC ColumnsExcelTMwww.ace-hplc.comACE Excel Ultra Robust UHPLC Columns5

C18Figure 6: Comparison of selectivity and characteristics ofACE ® Excel TM 2µm C18 to other manufacturers’ columnsApplication #1503 – Rapid UHPLC Screening of 16 Pharmaceuticals and Related CompoundsMechanisms ofSeparationStrength ofInteractionMobile Phase: A = 20 mM KH 2PO 4, pH 2.7 andB = 20 mM KH 2PO 4, pH 2.7 in MeOH/H 2O (65:35 v/v)Gradient: 3 to 100% B in 5 minutesFlow Rate: 0.6 ml/minTemperature: 60°CDetection: UV, 214 nmColumn Dimensions: 50 x 2.1mm31. N-acetylprocainamide2. 3-hydroxybenzoic acid3. pindolol4. methylphenylsulphoxide5. benzylalcohol6. quinoxaline7. 1,4-dinitrobenzene8. phenacetin9. 1,2-dimethoxybenzene10. furosemide11. anisole12. methylbenzoate13. remacemide14. nimesulide15. ethylbenzoate16. diflunisalHydrophobic bindinginteractionsShape selectivityStrongWeakACE Excel 2 C181245678910111612 14 1513P max= 309 barTarget AnalytesAnalytes differing in hydrophobicityPolar, moderately polar and nonpolar analytesUncharged acids and basesIonized acids or bases using ion-pairingZORBAX Eclipse1.8 XDB C18213456789101112 14 15 1613P max= 396 barRecommended ApplicationsAnalytes differing in hydrophobicityHomologous compounds differing by –CH2Ideal starting point for method development310, 11Waters ACQUITY1.7 BEH C1812456789121314 15 16P max= 478 bar3,4PhenomenexKinetex 1.7 C181,2567891110121314 1516P max= 446 bar012Minutes345Comparative data may not be representative of all applications.Please see p.10 for acknowledgement of trademarksFurther Information;An HPLC product brochure discussing ACE C18 and allACE phases is available.Contact your distributor to request your copy or visitwww.ace-hplc.com for further details.In this example the ACE Excel C18 UHPLC column provides retentionand selectivity similar to the other C18 UHPLC columns (althoughthere may be slight selectivity differences between different C18phases). Under these conditions, these slight differences allow the ACEExcel C18 to fully separate all 16 components of interest.As with all ACE HPLC columns, ACE Excel UHPLC columns also deliverexcellent peak shape for your analytes. Additionally, owing to theoptimal 2µm particle size and a rigorous classification protocol, backpressure for all ACE Excel UHPLC columns is significantly lower thanfor these leading UHPLC columns packed with

C18-AR(C18 with integral Phenyl functionality)Figure 7: Leveraging the unique ACE ® Excel TM C18-AR selectivityApplication #1504 – Water Soluble Vitamins by UHPLCMobile Phase: A = 20 mM KH 2PO 4, pH 2.7 andB = 20 mM KH 2PO 4, pH 2.7 in MeOH/H 2O (50:50 v/v)Gradient: 20 to 70% B in 5 minutesFlow Rate: 0.31 ml/minTemperature: 40°CDetection: UV, 205 nmColumn Dimensions: 50 x 2.1mm1. pyridoxine2. p-aminobenzoic acid3. pantothenic acid4. folic acid5. d-biotin6. cyanocobalamin7. riboflavinMechanisms ofSeparationπ-π interactionsDipole-dipole interactionsHydrophobic bindinginteractionsShape selectivityTarget AnalytesStrength ofInteractionStrongModerateStrongModerateACE Excel 2 C18-ARACE Excel 2 C181143 245,62 3567P max= 209 barP max= 216 barAnalytes with π bonding and conjugated systems7Analytes with electron delocalization and electronwithdrawinggroups, such as halogens, nitro groups,ketones, esters and acids4Analytes with different dipole momentsAnalytes differing in hydrophobicityPolar, moderately polar and nonpolar analytesZORBAX Eclipse1.8 XDB C18123567P max= 299 barUncharged acids and basesRecommended Applications4Analytes differing in hydrophobicity, homologouscompounds differing by –CH 2StereoisomersWaters ACQUITY1.7 BEH C1812 3 576P max= 336 barSteroidsSubstituted aromatics4Sulphur containing compoundsSuitable for use in up to 100% aqueous mobilephase conditionsParticularly recommended for applications where aC18 does not provide an adequate separationPhenomenexKinetex 1.7 C1812, 3567P max= 315 bar03MinutesComparative data may not be representative of all applications.Please see p.10 for acknowledgement of trademarks124 56This test illustrates that the leading C18 UHPLC column brands shownabove do not readily separate these water soluble vitamins under theconditions stated.Further Information;A product bulletin discussing the unique ACE C18-ARphase is now available.Contact your distributor to request your copy or visitwww.ace-hplc.com for further details.The additional mechanisms of separation provided by the ACEExcel 2µm C18-AR generate a better separation for both peakpairs, with resolution significantly improved under these conditions.Optimisation of both column dimensions and evaluation conditions,to further reduce analysis time, is now possible.www.ace-hplc.comACE Excel Ultra Robust UHPLC Columns7

C18-PFP(C18 with integral PFP functionality)Figure 8: Leveraging the unique ACE ® Excel TM C18-PFP selectivityApplication #1505 – Structural Isomers by UHPLCMobile Phase: MeOH/H 2O (50:50 v/v)Flow Rate: 0.21 ml/minTemperature: 40 °CColumn Dimensions: 50 x 2.1mm1. 1,2,3-trimethoxybenzene2. 1,2-dimethoxybenzene3. 1,2,4-trimethoxybenzene4. methoxybenzene5. 1,3-dimethoxybenzene6. 1,3,5-trimethoxybenzene7. toluene136Mechanisms ofSeparationStrength ofInteractionACE Excel 2 C18-PFP(fully porous ultra inert silica) 2457π-π interactionsStrongDipole-dipole interactionsStrongHydrophobic bindinginteractionsShape selectivityStrongStrongACE Excel 2 C18(fully porous ultra inert silica)1, 2, 364, 57Target AnalytesAnalytes with π bondingAnalytes with electron donating groups, such asphenols, aromatic ethers and aminesAnalytes with proton donor groupsAnalytes with different dipole momentsWaters ACQUITY 1.7BEH C18(hybrid particle)1, 2, 34, 567Analytes differing in hydrophobicityPolar, moderately polar and nonpolar analytes1, 2, 3Uncharged acids and basesRecommended ApplicationsAgilent Poroshell 2.7120 EC C18(superficially porous particle)4, 567Structural isomersSteroidsTaxanes1, 2, 3Substituted aromaticsAnalytes differing in hydrophobicity, homologouscompounds differing by –CH2Phenomenex Kinetex2.6 C18(core-shell particle)4, 567Suitable for use in up to 100% aqueous mobilephase conditionsParticularly recommended for applications where aC18 does not provide an adequate separationFurther Information;A product bulletin discussing the unique ACE C18-PFPphase is now available.Contact your distributor to request your copy or visitwww.ace-hplc.com for further details.0Comparative data may not be representative of all applications.Please see p.10 for acknowledgement of trademarks2MinutesThis test illustrates that under these conditions, the leading C18 UHPLCcolumn brands shown do not readily separate these structural isomersand that, irrespective of the nature of their particle construction, it isthe surface bonded phase effects that dominate. In particular, thehydrophobic binding interaction appears to offer no selectivity forseparating peaks 1, 2 and 3 and offers only poor resolution of peaks 4, 5and 6.The ACE Excel C18-PFP provides the additional selectivity needed toseparate the co-eluting analytes under these conditions. It is believedthat this additional selectivity is provided by a powerful combinationof the strong π-π and dipole-dipole interactions, coupled with thestrong shape selectivity and hydrophobic character offered by theunique C18-PFP phase.468ACE Excel Ultra Robust UHPLC Columnswww.ace-hplc.com

Phase Availability and SpecificationsPhase Functional Group Endcapped ParticleSize (µm)PoreSize (Å)SurfaceArea (m 2 /g)CarbonLoad (%)MaximumpH RangeUSPListing100% AqueousCompatibilityC18 Octadecyl (C18) Yes 2 a 100 300 15.5 1.5-10.0 b L1 -C18-AR C18 with integral Phenyl Yes 2 a 100 300 15.5 1.5-10.0 b L1 Yes CC18-PFP C18 with integral PFP Yes 2 a 100 300 14.3 1.5-10.0 b L1 Yes CC8 Octyl Yes 2 a 100 300 9.0 1.5-10.0 b L7 -C4 Butyl Yes 2 a 100 300 5.5 1.5-10.0 b L26 -CN Cyano Yes 2 a 100 300 5.5 2.0-8.0 b L10 -Phenyl Phenyl Yes 2 a 100 300 9.5 1.5-10.0 b L11 -AQ Proprietary Yes 2 a 100 300 14.0 1.5-10.0 b L1 Yes CSIL - - 2 a 100 300 - 1.5-8.0 b L3 -a ACE Excel UHPLC columns are also available with 3µm and 5µm particle sizes. ACE HPLC columns are also available with 3µm, 5µm and 10µm particle sizes.b For optimum column lifetime, a pH range of 2-8 is recommended. To increase column lifetime at higher pH, organic buffers, low buffer concentrations, high % organic solvent and low temperaturesmust be considered. Further information is contained within “A Guide to HPLC and LC/MS Buffer Selection” by John Dolan – contact your distributor to request your FREE copy.c These ACE phases may be used with up to 100% aqueous mobile phase without retention loss caused by pore dewetting/phase collapse effects.ACE ® UHPLC Reusable Column Connector• Pressure rating >1700bar (>25000psi)• Compatible with all UHPLC systems• Compatible with all UHPLC column brands• Eliminates Poor Connections• Unique Reusable DesignWrenchFlat 1/4”(0.64cm)Reusable columnconnector with10-32 threads for1/16” OD TubingProprietary PEEKfilled coneACE TorqueWrench EXL-TWFor further information and to receive a FREE productbulletin, please contact your local distributorAll UHPLC column brands require correct installation inorder to realise maximum column efficiency. To avoidproblems, the use of permanently swaged fittings is notrecommended, as these do not allow free movementbetween the tubing, fitting and column inlet on installation.This can result in a poorly connected column that showsunexpected peak tailing due to the introduction of extracolumn volume (dead volume) to the system. Alternatively,a leak at the inlet fitting connection may be observed.ACE UHPLC Column Connectors are reuseable and enableUHPLC columns to be correctly installed every time. Theirunique design ensures that they maintain pressure ratingwith repeated use, yet do not permanently swage onto theinlet tubing. To maximise the lifetime of the fitting, the useof an ACE Torque Wrench (p/n EXL-TW) is required.Standard ACE HPLC PEEK finger-tight connectors (p/n ACE-FT, pressure rated to 350bar/5000psi) may be used at theoutlet end of the UHPLC column, where pressure demandsare lower but a correct connection remains important.ACE Excel 2µm C18 ColumnsColumnDiameter2.1mm3.0mm4.6mmACE Excel 2µm C18-AR ColumnsColumnDiameter2.1mm3.0mm4.6mmACE Excel 2µm C18-PFP ColumnsColumnDiameter2.1mm3.0mm4.6mmColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mmEXL-101-0202UEXL-101-0203UEXL-101-0246UColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mmEXL-109-0202UEXL-109-0203UEXL-109-0246UColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mmEXL-1010-0202UEXL-1010-0203UEXL-1010-0246UEXL-101-0302UEXL-101-0303UEXL-101-0346UEXL-109-0302UEXL-109-0303UEXL-109-0346UEXL-1010-0302UEXL-1010-0303UEXL-1010-0346UEXL-101-3502UEXL-101-3503UEXL-101-3546UEXL-109-3502UEXL-109-3503UEXL-109-3546UEXL-1010-3502UEXL-1010-3503UEXL-1010-3546UEXL-101-0502UEXL-101-0503UEXL-101-0546UEXL-109-0502UEXL-109-0503UEXL-109-0546UEXL-1010-0502UEXL-1010-0503UEXL-1010-0546UEXL-101-7502UEXL-101-7503UEXL-101-7546UEXL-109-7502UEXL-109-7503UEXL-109-7546UEXL-1010-7502UEXL-1010-7503UEXL-1010-7546UEXL-101-1002UEXL-101-1003UEXL-101-1046UEXL-109-1002UEXL-109-1003UEXL-109-1046UEXL-1010-1002UEXL-1010-1003UEXL-1010-1046UEXL-101-1202UEXL-101-1203UEXL-101-1246UEXL-109-1202UEXL-109-1203UEXL-109-1246UEXL-1010-1202UEXL-1010-1203UEXL-1010-1246UEXL-101-1502UEXL-101-1503UEXL-101-1546UEXL-109-1502UEXL-109-1503UEXL-109-1546UEXL-1010-1502UEXL-1010-1503UEXL-1010-1546UUHPLCConnector1, 2, 3EXL-CC1, 2, 3EXL-CC1, 2, 3EXL-CCUHPLCConnector1, 2, 3EXL-CC1, 2, 3EXL-CC1, 2, 3EXL-CCUHPLCConnector1, 2, 3EXL-CC1, 2, 3EXL-CC1, 2, 3EXL-CC1 Also available as a 10pk p/n EXL-CC102 Torque wrench (p/n EXL-TW) is required3Starter kit available (p/n EXL-CCSK) containing 4x EXL-CC plus 1x EXL-TWwww.ace-hplc.comACE Excel Ultra Robust UHPLC Columns9

ACE Excel 2µm C8 ColumnsColumnDiameter2.1mm3.0mm4.6mmACE Excel 2µm C4 ColumnsColumnDiameter2.1mm3.0mm4.6mmACE Excel 2µm CN ColumnsColumnDiameter2.1mm3.0mm4.6mmACE Excel 2µm Phenyl ColumnsColumnDiameter2.1mm3.0mm4.6mmACE Excel 2µm AQ ColumnsColumnDiameter2.1mm3.0mm4.6mmColumnDiameter2.1mm3.0mm4.6mmColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mmEXL-102-0202UEXL-102-0203UEXL-102-0246UColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mmEXL-103-0202UEXL-103-0203UEXL-103-0246UColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mmEXL-104-0202UEXL-104-0203UEXL-104-0246UColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mmEXL-105-0202UEXL-105-0203UEXL-105-0246UColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mmEXL-106-0202UEXL-106-0203UEXL-106-0246UEXL-103-0302UEXL-103-0303UEXL-103-0346UEXL-104-0302UEXL-104-0303UEXL-104-0346UEXL-105-0302UEXL-105-0303UEXL-105-0346UEXL-106-0302UEXL-106-0303UEXL-106-0346UEXL-103-3502UEXL-103-3503UEXL-103-3546UEXL-104-3502UEXL-104-3503UEXL-104-3546UEXL-105-3502UEXL-105-3503UEXL-105-3546UEXL-106-3502UEXL-106-3503UEXL-106-3546UEXL-103-0502UEXL-103-0503UEXL-103-0546UEXL-104-0502UEXL-104-0503UEXL-104-0546UEXL-105-0502UEXL-105-0503UEXL-105-0546UEXL-106-0502UEXL-106-0503UEXL-106-0546UEXL-103-7502UEXL-103-7503UEXL-103-7546UEXL-104-7502UEXL-104-7503UEXL-104-7546UEXL-105-7502UEXL-105-7503UEXL-105-7546UEXL-106-7502UEXL-106-7503UEXL-106-7546UEXL-103-1002UEXL-103-1003UEXL-103-1046UEXL-104-1002UEXL-104-1003UEXL-104-1046UEXL-105-1002UEXL-105-1003UEXL-105-1046UEXL-106-1002UEXL-106-1003UEXL-106-1046UEXL-103-1202UEXL-103-1203UEXL-103-1246UEXL-104-1202UEXL-104-1203UEXL-104-1246UEXL-105-1202UEXL-105-1203UEXL-105-1246UEXL-106-1202UEXL-106-1203UEXL-106-1246UEXL-103-1502UEXL-103-1503UEXL-103-1546UEXL-104-1502UEXL-104-1503UEXL-104-1546UEXL-105-1502UEXL-105-1503UEXL-105-1546UEXL-106-1502UEXL-106-1503UEXL-106-1546UColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mmEXL-107-0202UEXL-107-0203UEXL-107-0246UEXL-102-0302UEXL-102-0303UEXL-102-0346UACE Excel 2µm SIL ColumnsEXL-107-0302UEXL-107-0303UEXL-107-0346UEXL-102-3502UEXL-102-3503UEXL-102-3546UEXL-107-3502UEXL-107-3503UEXL-107-3546UEXL-102-0502UEXL-102-0503UEXL-102-0546UEXL-107-0502UEXL-107-0503UEXL-107-0546UEXL-102-7502UEXL-102-7503UEXL-102-7546UEXL-107-7502UEXL-107-7503UEXL-107-7546UEXL-102-1002UEXL-102-1003UEXL-102-1046UEXL-107-1002UEXL-107-1003UEXL-107-1046UEXL-102-1202UEXL-102-1203UEXL-102-1246UEXL-107-1202UEXL-107-1203UEXL-107-1246UEXL-102-1502UEXL-102-1503UEXL-102-1546UEXL-107-1502UEXL-107-1503UEXL-107-1546UUHPLCConnector1, 2, 3EXL-CC1, 2, 3EXL-CC1, 2, 3EXL-CCUHPLCConnector1, 2, 3EXL-CC1, 2, 3EXL-CC1, 2, 3EXL-CCUHPLCConnector1, 2, 3EXL-CC1, 2, 3EXL-CC1, 2, 3EXL-CCUHPLCConnector1, 2, 3EXL-CC1, 2, 3EXL-CC1, 2, 3EXL-CCUHPLCConnector1, 2, 3EXL-CC1, 2, 3EXL-CC1, 2, 3EXL-CCUHPLCConnector1, 2, 3EXL-CC1, 2, 3EXL-CC1, 2, 3EXL-CC1 Also available as a 10pk p/n EXL-CC102 Torque wrench (p/n EXL-TW) is required3Starter kit available (p/n EXL-CCSK) containing 4x EXL-CC plus 1x EXL-TWACE ® is a registered trademark of Advanced Chromatography Technologies Limited. ACE Excel TM and HSC TM are trademarks of Advanced Chromatography Technologies Limited.UPLC, XBridge and ACQUITY are trademarks of Waters Corporation; ZORBAX, Eclipse, Poroshell and Infinity are trademarks of Agilent Technologies Inc.; Kinetex is atrademark of Phenomenex Inc.; UltiMate and Accela are trademarks of Thermo Fisher Scientific; Nexera and Prominence are trademarks of Shimadzu Corporation.Advanced Chromatography Technologies Limited has no affiliation with Waters Corporation, Agilent Technologies Inc., Phenomenex Inc., Thermo Fisher Scientific orShimadzu Corporation.ACE products are available through our international distributor networkAvailable from:ExcelTMUHPLC Columnswww.ace-hplc.com10Advanced Chromatography Technologies Limited, 1 Berry Street, Aberdeen, AB25 1HF, ScotlandTel: +44 (0) 1224 704 554 Fax: +44 (0) 1224 841 301 email: info@ace-hplc.com web: www.ace-hplc.comP1155-12-11-003-14000