Agenda Item 16 CX/CF 13/7/16 February 2013 JOINT FAO ... - FAO.org

CX/CF 13/7/16 12Table XX: Analytical methods for the determination of methylmercury in fish used by Codex membersCountry Method Summary of method Principle Validation study ApplicabilityMinimumapplicablerange (mg/kg)LOD,LOQ(mg/kg)Recovery(%)RSDRCanadaHPLC-ICP-MSmethod formercury andmethylmercuryspeciationanalysis in fishFish samples extractedwith 0.75% HclL-Cysteine andanalyzed on an AgilentICP/MS 7500equipped with aMicromist nebulizerand a Scott typenebulisation chambercooled at 2◦C followedby separation onHPLCHPLC-ICP-MSTwo laboratorycomparison usingtwo differentHPLC systemsFish LOD: Hg: 0.002MeHg: 0.005LOQ: Hg: 0.006MeHg: 0.015 (3XLOD)Hg: 103±2.1%MeHg:99±2.1%Hg: 2.1%MeHg: 2.1%ChinaDetermination ofMethylmercuryand TotalMercury inSeafoodMethylmercury in thesamples is extractedby HCl andcysteine-aqueoussolution. Then it isseparated by LC anddetected by AtomicFluorescenceSpectrometer (AFS)LC-AFS Seafood LOD: 0.002LOQ: 0.008JapanAcidleaching-benzeneextraction withGC-ECDMethylmercury in thesamples is extractedinto benzene afteracidification byHCl(cysteine-aqueoussolution is used forback-extraction).Methylmercury isseparated by GC anddetected by ECDGC-ECD Single-laboratory Seafood 0.01-5.0 MeHg LOQ: 0.01 MeHg 70-120 -