ab83389 Glutamate Assay Kit - Abcam

The Glutamate Enzyme Mix is stable for up to 2 months at -20°Cafter reconstitution or freeze-thaw cycle less than 5 times.b) Reconstitute Glutamate Developer with 820 μl of ddH 2 O. Pipetteup and down several times to completely dissolve the pellet intosolution (Don’t vortex).c) Mix enough reagents for the number of samples and standards tobe performed. For each well, prepare a total 100 μl Reaction Mix:Reaction Mix Bkgd Control MixAssay Buffer 90 µl 92 µlGlutamate Developer 8 µl 8 µlGlutamate Enzyme Mix 2 µl --Add 100 μl of the Reaction Mix to each well containing theGlutamate Standard and test samples. To the background controlwell, add 100 µl of background control mix. Mix well. Incubate at37°C for 30 minutes, protect from light.4. Measure OD at 450 nm in a microplate reader.8