Duopath Legionella - mibius

Duopath ®LegionellaFor the rapid detection of Legionella species and Legionellapneumophila in water and environmental samples

Duopath ® LegionellaGLISA-Rapid Test (Gold Labelled ImmunoSorbent Assay) for thequalitative simultaneous detection of Legionella spp. and Legionellapneumophila in water and environmental samples.Legionella are gram-positive, rod-shaped bacteria, whichare present in nearly all aquatic biotopes of fresh water.They are frequently associated with Protozoans, e.g.amoebae, where they can grow intracellular. In most casesLegionella is present in relatively low numbers in drinkingwater. Thus Legionella usually present no health risk tohumans. However, special environmental conditions, e.g.water temperatures between 30°C and 45°C, longer waterretention times (e.g. cooling towers, containments ofhot water or water pipes which have been unused for a longperiod) promote vigorous growth of these bacteria.Infections caused by Legionella occur via inhalation ofLegionella-contaminated aerosols, e.g. while showering, inswimming-and bathing pools (especially whirlpools), aswell as via air conditioning systems.After inhalation Legionella is able to grow in pulmonarymacrophages causing a serious, life-threatening andatypical Pneumonia, the so called Legionnaires’ Disease.At least 42 different Legionella species are known. It issupposed that not only the well-known species Legionellapneumophila but all species can cause Legionnaires’Disease.Within the microbiological analysis of water samples notonly the presence of Legionella pneumophila must betested but also the genus Legionella should be included.The presence of Legionella in water samples is an indicatorof concerning hygienic conditions in aquatic systems.The drastic increase of outbreaks of Pneumonia caused byLegionella in public buildings demands reliable and rapiddetection methods. Besides the traditional culture methods,immunological techniques are preferred by users due tothe higher sensitivity and specificity.Duopath® Legionella is a reliable, rapid, cost-effectiveimmunological test based on the immune flow principle.It is designed for the simultaneous detection of Legionellaspp. and Legionella pneumophila in water and environmentalsamples in such a way that time-consuming andpersonnel intensive working steps are avoided.Your benefitsReliableFastEase-of-useConvenientSafeEconomicalAs specific as the ELISA method. Provides moreaccurate results.Result in just 20-30 minutes.One-step format avoids working errors during handling.Simply add sample and read off the result.Clear and distinct positive or negative testresults with a built-in positive control.Rapid results save labour and inventory costs.No capital investment required for example forinstrumentation such as automated systems.

Flow-diagram of Duopath ® GLISA Legionella test procedure• Plate water sample directly onto BCYEagar or GVPC agar• Filter 10ml to 1000ml of the watersample and treat accordingly• Place filter directly on BCYE agar orGVPC agar• Incubate for up to 10 days at 36 ±2°C• Pick up 1-3 typical colonies (min. ø 1mm,max. ø 3mm) from the BCYE agar or GVPCagar with a wooden toothpick and dispense itin 144μl of the 0.9% NaCl/1% -Tween®20 -Stock solution in an Eppendorf cup• Add 16 μl Polymyxin stock solution and mix• Incubate for 2-5 min at room temperature(+15 to +25°C).• Heat up and incubate at 95°C for 5min• Allow to cool to room temperatureEnrichment• Transfer 150 μl to test deviceand read result after30 min.positiveLegionella spp.presentnegativeLegionellanot presentLegionellapneumophilapresentDetectionProduct listProducts Pack size Ord.No.Bacillus cereus Selective Supplement (Polymyxin B-sulfate) 16 vials 1.09875.0001Sodium chloride 5 kg 1.06400.5000Tween® 20 (Polysorbate) 1l 8.17072.1000Duopath® Legionella 25 tests 1.04147.0001Legionella Combi Pack 3x6 vials 1.10425.0001Legionella CYE Agar base 12 vials 1.10242.0001Legionella BCYE -Growth Supplement 5 vials 1.10240.0001Legionella GVPC Selective Supplement 4 vials 1.10241.0001Merckoplate Legionella GVPC 20 plates 1.00098.0020Merckoplate Legionella BCYE 20 plates 1.10097.0020EnrichmentDetectionIsolation media

Lateral flow testsFor the rapid detection ofpathogens in foodSame safety standard as the classical detection method:Simple to perform, reliable results in just 20 minutes, considerablesavings in time and costs.Wider product range:Lateral flow tests detect important pathogens in food and environment:E.coli 0157, Verotoxin-producing E.coli, Salmonella, Campylobacter, Listeria,Listeria monocytogenes, Legionella and enterotoxinogenic Bacillus cereus.Additional plus:Especially adapted media for precise and reliable results.W286145 02/06Merck KGaA64271 Darmstadt, GermanyFax: +49 (0) 61 51/72 60 80Email: microbiology@merck.dewww.merck.demicrobiology.merck.deWe provide information and advice to our customers to the best of our knowledge and ability, but without obligation or liability. Existing laws andregulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do notrelieve our customers of their own responsibility for checking the suitability of our products for the envisaged purpose.Duopath® is a registered trademark of Merck KGaA, Darmstadt, Germany.