T: Mitos Duo XS-Pump Part No. 3200057 - Dolomite Microfluidics

The Dolomite Centre LtdUnit 1, Anglian Business Park, Orchard Road,Royston, Hertfordshire, SG8 5TW, UKT: +44 (0)1763 242491F: +44 (0)1763 246125E: sales@dolomite-microfluidics.comW: www.dolomite-microfluidics.comMitos Duo XS-PumpPart No. 3200057Dolomite Microfluidics – North America OfficeBlacktrace Inc, 29 Albion PlaceCharlestown, MA 02129, USAC: 617 803 6655T: 617 848 1121F: 617 500 0136E: salesus@dolomite-microfluidics.comproduct datasheetpageDescription 2Benefits 2Specifications 3Mitos Duo XS-Pump Flow Rates 3User Interface and Control 4Modes of Operation 5Extra Smooth Pumping 6Mitos Duo XS Pump Basic 7Contents 7Mitos Duo XS-Pump Basic Flow Rates 8Parts and Accessories 8The Dolomite Centre Limited Page 1 of 10

The Dolomite Centre LtdDescriptionThe Mitos Duo XS-Pump (Part No. 3200057) is a versatile microfluidic pumping solution.It can be configured into 2 refilling flows or 1 continuous flow. It is operated using anintuitive twist and click knob and display or by a PC using free software. A choice of flowmodes allows simple or complex flow control.Left: Mitos Duo XS-Pump(Part No. 3200057).Above: User replaceable syringesallow wide flow rate range. Partnumbers are given on Page 7.Benefits• Versatile with many modes of operation• Advanced drive electronics• Easy to use• Small footprint• Extra smooth pumping eliminates pulsation issues• Wide temperature / pressure range• Wide flow rate range - syringes are easily replaceable• Excellent chemical compatibilityMitos Duo XS-Pump_product datasheet Page 2 of 10

The Dolomite Centre LtdSpecificationsPump SpecificationNumber of channelsValue2 refilling or 1 continuousNumber of input streams 1 - 10Number of output streams 1 - 10Flow rate rangeOperating pressure rangeSize (height x width x depth)Wetted materials0.1µl/min - 30ml/min0 - 6Bar (0 - 90PSI)260mm x 160mm x 250mmGlass, PTFE and PCTFEMitos Duo XS-Pump Flow RatesThe Mitos Duo XS-Pump allows flow rates in both dual and continuous mode.Dual ModeSyringe Size (µl) Min Syringe Speed (µl/min) Max Syringe Speed (µl/min).50 0.1 300100 0.2 600250 0.5 1500500 1 30001000 2 60002500 5 150005000 10 30000Mitos Duo XS-Pump_product datasheet Page 3 of 10

The Dolomite Centre LtdContinuous ModeSyringe Size (µl) Min Syringe Speed (µl/min) Max Syringe Speed (µl/min).50 0.1 176100 0.2 352250 0.5 882500 1 17641000 2 35292500 5 88205000 10 17647The max syringe speed in continuous mode takes into account an estimated valveswitching time on the fill stroke of a syringe.User Interface and ControlThe Mitos Duo XS-Pump can be used on its own with the twist and click control knob andlarge display. Automated liquid reagent addition may be carried out by setting dispensevolume or flow rate over a time period. It is also possible to generate advanced flow rateprofiles.Mitos Duo XS-Pump_product datasheet Page 4 of 10

The Dolomite Centre LtdModes of OperationThe pump has two syringes and two multi-port valves allowing it to be configured in anumber of ways. As each port can be used to aspirate from or dispense to, the pump canbe used as “one to many”, “many to one” or one continuous flow. The variousconfiguration options using a 6-port valve are shown below:10 liquid reagents to1 microfluidic device10 liquid reagents to2 microfluidic devices1 liquid reagent to 10microfluidic devices2 liquid reagents to 10microfluidic devicesMixing reagents withfeedback controlContinuous reagentadditionMitos Duo XS-Pump_product datasheet Page 5 of 10

The Dolomite Centre LtdExtra Smooth PumpingThe Mitos Duo XS-Pump uses advanced electronics to remove the pulsation from thestepper motor drive, providing pulse-free flow. This is required for microfluidic applicationswhere pulsation from the stepper motor can affect performance.The graphs below show the flow performance that can be obtained using the Duo XS-Pump with a 12.5µl glass syringe.1.4Response time test1.2Flow rate (ul/min)10.80.60.40.200 5 10 15Time (seconds)1.2Flow rate accuracy test1Flow rate (ul/min)0.80.60.40.200 5 10 15 20 25 30 35 40 45 50Time (seconds)1.2Gradient test1Flow rate (ul/min)0.80.60.40.200 5 10 15 20 25 30 35 40 45 50 55 60Time (seconds)Notes: The graphs show flow rate (raw data) measured using a thermal anemometer sensor. Prior to the testssome optimisation was carried out to match the response of the pump to the fluidic system.Mitos Duo XS-Pump_product datasheet Page 6 of 10

The Dolomite Centre LtdApplication examples1. Droplet generation in a microfluidic chip. In this application one channel pumped oiland the other channel pumped water into a microfluidic chip. A T-junction was used toproduce a stream of 100 micron diameter droplets.2. Surface modification of microfluidic devices. In this application a pump channel wasconnected to a microfluidic device and series of reagents were pumped through thedevice. The flow rates and residence times were adjusted to optimise the surfacemodification procedure.Mitos Duo XS-Pump BasicThe Mitos Duo-XS Pump Basic (Part No. 3200066)is operated via free PC software or via your ownsoftware using the user API (it does not include atwist and click knob and display). All the otherfeatures on the pump are the same including extrasmooth pumping, wide flow rate range and thevarious modes of operation.ContentsThe pumps will be supplied with the following items:• User manual• 24V Power supply• Decontamination CertificateNote: 2 Valves and 2 syringes will be required from the Parts and Accessories list to usethe pump.Mitos Duo XS-Pump_product datasheet Page 7 of 10

The Dolomite Centre LtdMitos Duo XS-Pump Basic Flow RatesThe Mitos Duo XS-Pump Basic allows flow rates in dual mode.Dual ModeSyringe Size (µl) Min Syringe Speed (µl/min) Max Syringe Speed (µl/min).50 0.1 300100 0.2 600250 0.5 1500500 1 30001000 2 60002500 5 150005000 10 30000Continuous ModeThe Mitos Duo XS-Pump Basic can support continuous flow if user programs this modevia the user software with the user API provided.Parts and AccessoriesVarious valves are available depending on the number of inputs and outputs required.Dolomite offers the following accessories to be used with the Mitos Duo XS-Pump andMitos Duo XS-Pump Basic:4-Port ValveTwo user replaceable syringesMitos Duo XS-Pump_product datasheet Page 8 of 10

The Dolomite Centre LtdThe Mitos XS-Pump Starter KitUSB to RS232 Adaptor CableDescription Specification Part No.2 Port Valve (PTFE/PCTFE) Max. Pressure = 6bar 30002443 Port Valve (PTFE/PCTFE) Max. Pressure = 6bar 30002454 Port Valve (PTFE/PCTFE) Max. Pressure = 6bar 30002466 Port Valve (PTFE/PCTFE) Max. Pressure = 6bar 3000247Syringe for Mitos Duo XS-Pump - 50µl Typical flow rates: 0.1µl/min to 300µl/min 3000248Syringe for Mitos Duo XS-Pump - 100µl Typical flow rates: 0.2µl/min to 600µl/min 3000249Syringe for Mitos Duo XS-Pump - 250µl Typical flow rates: 0.5µl/min to 1500µl/min 3000250Syringe for Mitos Duo XS-Pump - 500µl Typical flow rates: 1µl/min to 3ml/min 3000251Syringe for Mitos Duo XS-Pump - 1ml Typical flow rates: 2µl/min to 6ml/min 3000252Syringe for Mitos Duo XS-Pump - 2.5ml Typical flow rates: 5µl/min to 15ml/min 3000253Syringe for Mitos Duo XS-Pump - 5ml Typical flow rates: 10µl/min to 30ml/min 3000254The Mitos XS-Pump Starter KitKrytox GPL 205 Fluorinated GreaseContains a selection of tubing and fittings toenable fluidic connections between a MitosXS-Pump and a microfluidic system.Available in 0.5oz, this Fluorinated Grease isideal for lubricating the syringes used withthe syringe pumps. This is required whenpumping some organic liquids, in particularhydrocarbons and chlorinated hydrocarbons.30003353200163USB to RS232 Adaptor Cable Connection to a PC 3200197Note that the pump is not limited to the typical flow rates. At the lower end of the flow rate rangeflow stability is reduced and the use of a smaller syringe size is recommended.Mitos Duo XS-Pump_product datasheet Page 9 of 10

The Dolomite Centre LtdMitos Duo XS-Pump_product datasheet Page 10 of 10