TENDER FOR PROCUREMENT OF LABORATORY EQUIPMENTS ...
TENDER FOR PROCUREMENT OF LABORATORY EQUIPMENTS ... TENDER FOR PROCUREMENT OF LABORATORY EQUIPMENTS ...
Tea Board/Kolkata/2011-12- QCL Tender Document-Originale.HPLC SPECIFICATIONSHigh Performance Liquid Chromatograph with Poly Diode Array (PDA), Fluorescence detectorwith autosampler, online degasser unit, column holding chamber and software aided fullyautomatic operation is required for analysis of antibiotics, aflatoxins, amino acids and otherchemical parameters etc. The equipment and its accessories are to be operated through Interfacecontrolled by software. All the components of HPLC have to be communicated throughcommunication ports and Interface which then controlled by software. The latest configurationPC, LCD/LED monitor and Laserjet printer etc are to be provided along with the abovementioned equipment.Solvent Delivery UnitSlNoSpecifications/RequirementsCriterion1 PumpBinary/Quarternary Gradient with Dynamicgradient mixerShould be capable of operating with 3-4Solvents at a time during gradient operation2 Composition Range 0 – 100 % among the 3 - 4 solvent lines3 Gradient mixing accuracy ± 0.5 %4 Gradient curve Linear and Exponential (Positive and Negative)5 Flow range 0.1 to 5 ml/min or better6 Flow Increment 0.1 ml7 Flow accuracy ± 1 %8 Flow Precision RSD < 0.5 % or better9 Retention time reproducibility RSD < 0.5 % or better10 Pumping System Software controlled11 Maximum Pressure 5000 Psi or better12 PulngerPump should be micro-volume double plungerpumpCapacity : 10 ul or Specify13 Plunger Rinsing mechanismAutomatic1415pH of operation 1 – 9Operatingcondition16 Safety MeasuresenvironmentalTemp : 22 – 40 o C and Humidity – 20 -80 %RHSensors for leak detection and auto cut off athigh pressure should be provided as safetymeasure37
Tea Board/Kolkata/2011-12- QCL Tender Document-Original17 Communication Port AvailableDegasserSlNoSpecifications/Requirements1 DegasserCriterion2 Maximum flow rate 10 ml/ minOnline VacuumNo of degassed solvents should be 3 – 4 anddegasser flow line capacity 300- 500 ul3 Number of channels4 - 5 (3 - 4 for line and 1 for autosampler rinsing)or specify4 Internal volume Typically 12 ml per channel5Material in contact withsolventPTFE, PEEK6 Communication Port AvailableAuto samplerSlNoSpecifications/Requirements1 Tray CapacityCriterion2 Replicate injections 1 - 99 injections3 Vial Detection Through SensorNot Less than 50 vials toaccommodate 1.5 – 2 ml vials/ tubesand 3 - 5 Nos of 10 ml vials for rinsingand dilution options4 Injection Volume RangeTotal sample injection or variableinjection volume (no sample loss)5 Loop volume Specify6 Injection volume accuracy ± 1 %7 Injection volume repeatability ≤ 0.5 % RSD8 Injector Linearity >0.999 ( r²)9 Sample Syringe Sizes 250 μl or able to inject 5–50 ul volume10 Injection Cycle time 2 – 3 samples per minute11 Sample Probe Pressure Assisted injection12 Needle RinsingBefore and after each injection, outerand inner of needle38
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Tea Board/Kolkata/2011-12- QCL Tender Document-Original17 Communication Port AvailableDegasserSlNoSpecifications/Requirements1 DegasserCriterion2 Maximum flow rate 10 ml/ minOnline VacuumNo of degassed solvents should be 3 – 4 anddegasser flow line capacity 300- 500 ul3 Number of channels4 - 5 (3 - 4 for line and 1 for autosampler rinsing)or specify4 Internal volume Typically 12 ml per channel5Material in contact withsolventPTFE, PEEK6 Communication Port AvailableAuto samplerSlNoSpecifications/Requirements1 Tray CapacityCriterion2 Replicate injections 1 - 99 injections3 Vial Detection Through SensorNot Less than 50 vials toaccommodate 1.5 – 2 ml vials/ tubesand 3 - 5 Nos of 10 ml vials for rinsingand dilution options4 Injection Volume RangeTotal sample injection or variableinjection volume (no sample loss)5 Loop volume Specify6 Injection volume accuracy ± 1 %7 Injection volume repeatability ≤ 0.5 % RSD8 Injector Linearity >0.999 ( r²)9 Sample Syringe Sizes 250 μl or able to inject 5–50 ul volume10 Injection Cycle time 2 – 3 samples per minute11 Sample Probe Pressure Assisted injection12 Needle RinsingBefore and after each injection, outerand inner of needle38
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