an investigation of domestic laundry in europe - habits ... - SIFO

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80An investigation into the domestic laundry habits in EuropeBacillus cereus colonies are pink and surrounded by a zone of precipitation.Suspected colonies are plated on MYP/PREP and again incubated for one dayat 30°C. The result is considered positive if the colonies are pink and surroundedby a zone of precipitation. It is also possible to see Enterococcus faecalison these plates. These colonies are yellow, glossy and sized 0.5mm.Enumeration of the Total Plate Count1 ml of the serial dilution is transferred to a plate and mixed with ca 15mlPCA (Plate-Count-Agar, Merck, 5463). The plates are incubated for threedays at 30°C. Every visible colony on this plate is counted.Enumeration of yeast and fungi0.1ml of the serial dilution is spread on an OGGA-plate (BiotradingK1042090) and incubated for 5-7 days at 25°C. During this period the platesare checked on the third day and the yeasts are counted. This is done in orderto see the yeasts before they are overgrown by fungi.4.3.8 CalculationThe number of micro-organisms is calculated according to normal microbiologicalrules.NNΣan 1n 2d∑ a=( n + 0, n ) d112= number of colonies= sum of the number of counted colonies= number of plates in first dilution= number of plates in second dilution (less diluted)= dilution rate corresponding first dilutionPlates with one or two colonies are considered negative, as these findingsmight be due to cross-contamination during the test. A negative result is writtenas
Hygiene effects of laundry processes in Europe 814.4 ResultsIn this chapter, the results of the assessment of the hygienic quality (i.e. themicrobiological count) of naturally soiled laundry will be presented beforeand after washing for four different European countries. Most results aregraphically depicted in bar charts where a comparison of the microbiologicalcount before washing and after washing is made. The X-axis indicates thewash temperature and the situation before washing. The Y-axis gives the logarithmicmicrobiological count per 4.7 cm 2 .4.4.1 Washing at low temperaturesThe results of the microbiological counts for the 15°C and 30°C programmeare shown in graphs 4.1 and 4.2. The colony counts are summarised in appendixesC and D. The data have been statistically analysed. In the analysis, forpractical reasons it is assumed that the data values
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