an investigation of domestic laundry in europe - habits ... - SIFO

an investigation of domestic laundry in europe - habits ... - SIFO an investigation of domestic laundry in europe - habits ... - SIFO

12.07.2015 Views

98An investigation into the domestic laundry habits in Europewith the average laboratory results. The results also show that the variationsbetween the different household washes are very small. Statistical analysis didnot show any differences between the average laboratory test results and theresults of the household washing processes (ANOVA-One Way, α = 0.05).The average results of the laboratory test at 60°C are again based on testsfrom Greece, Norway and the Netherlands. The results of the two processeswith bleaching agents (average results of the laboratory test and wash G) arehighly comparable. The colony count of the washing process without bleachingagents is, as expected, somewhat higher.Table 4-7 Hygienic quality of sterile samples after washing at 60°C [logarithmic count ofCFU in 4.7 cm2 sample]average results laboratorywash Fwash Gtestswith bleach without bleach with bleachTotal Plate Count 2.2 3.0 2.1Enterobacteriaceae

Hygiene effects of laundry processes in Europe 99quality of the most common washing processes in Greece, Norway and theNetherlands is more or less comparable. The reduction on the diaper samplevaried between 3 and 5.5 log units. About log 1.9 to 4.4 micro-organisms percm 2 remained after washing. The results from the Spanish washing processesseem to be very different from the other European countries. Only log 0.6 andlog 2 micro-organisms per cm 2 remained on a washed diaper. This indicates ahigher reduction level. The tests showed that the hygienic quality depends onthe washing temperature and the presence of bleaching agents in the detergentas well as on the interaction between these factors. This is confirmed by anadditional test. Almost all micro-organisms were removed from the laundrysamples in a 95°C programme using a detergent containing bleach.An interesting question is how these results compare to the hygienic quality ofevery day clean laundry. The hygienic quality is evaluated directly after washingwhile the samples are still wet. On clean, dry laundry the Total PlateCount is considerably lower. Samples taken from clean laundry in a cupboardshow that the average count is about log 2.5 CFU /4.7cm 2 . Drying and ironingseem to have a positive effect on the amount of micro-organisms. One of themost interesting findings is that washing seems to spread micro-organismsrather than removing them. Sterile samples were very often contaminated withall kinds of bacteria. But the sterile samples are not the only indication ofcross-contamination and the spreading of micro-organisms. Spreading is alsofound among laundry items. For example, Enterobacteriaceae were foundonly on the unwashed diaper and dishcloth, but all other items were contaminatedafter washing.Another interesting outcome of this research is the increase in hygienic qualityof the laundry items achieved by adding an extra rinse to the washingprocess. This finding emerged when verifying the Spanish results. The Spanishhygiene level seems to be systematically higher than in the other Europeancountries. One explanation for this could be the extra rinse, which was addedto the wash cycle due to extensive foaming. In an additional experiment theeffect of an extra rinse was tested. The hygienic quality was improved bymore than 1 log unit.4.6.1 Evaluation of the test methodThe repeatability and validation of the method developed were evaluated inChapter 4.6.1 and 4.6.2 The results showed that the method seems to be repeatable,is valid and reflects common daily practice. However, before bringingthis method to the attention of standardisation bodies, research institutes,

Hygiene effects <strong>of</strong> <strong>laundry</strong> processes <strong>in</strong> Europe 99quality <strong>of</strong> the most common wash<strong>in</strong>g processes <strong>in</strong> Greece, Norway <strong>an</strong>d theNetherl<strong>an</strong>ds is more or less comparable. The reduction on the diaper samplevaried between 3 <strong>an</strong>d 5.5 log units. About log 1.9 to 4.4 micro-org<strong>an</strong>isms percm 2 rema<strong>in</strong>ed after wash<strong>in</strong>g. The results from the Sp<strong>an</strong>ish wash<strong>in</strong>g processesseem to be very different from the other Europe<strong>an</strong> countries. Only log 0.6 <strong>an</strong>dlog 2 micro-org<strong>an</strong>isms per cm 2 rema<strong>in</strong>ed on a washed diaper. This <strong>in</strong>dicates ahigher reduction level. The tests showed that the hygienic quality depends onthe wash<strong>in</strong>g temperature <strong>an</strong>d the presence <strong>of</strong> bleach<strong>in</strong>g agents <strong>in</strong> the detergentas well as on the <strong>in</strong>teraction between these factors. This is confirmed by <strong>an</strong>additional test. Almost all micro-org<strong>an</strong>isms were removed from the <strong>laundry</strong>samples <strong>in</strong> a 95°C programme us<strong>in</strong>g a detergent conta<strong>in</strong><strong>in</strong>g bleach.An <strong>in</strong>terest<strong>in</strong>g question is how these results compare to the hygienic quality <strong>of</strong>every day cle<strong>an</strong> <strong>laundry</strong>. The hygienic quality is evaluated directly after wash<strong>in</strong>gwhile the samples are still wet. On cle<strong>an</strong>, dry <strong>laundry</strong> the Total PlateCount is considerably lower. Samples taken from cle<strong>an</strong> <strong>laundry</strong> <strong>in</strong> a cupboardshow that the average count is about log 2.5 CFU /4.7cm 2 . Dry<strong>in</strong>g <strong>an</strong>d iron<strong>in</strong>gseem to have a positive effect on the amount <strong>of</strong> micro-org<strong>an</strong>isms. One <strong>of</strong> themost <strong>in</strong>terest<strong>in</strong>g f<strong>in</strong>d<strong>in</strong>gs is that wash<strong>in</strong>g seems to spread micro-org<strong>an</strong>ismsrather th<strong>an</strong> remov<strong>in</strong>g them. Sterile samples were very <strong>of</strong>ten contam<strong>in</strong>ated withall k<strong>in</strong>ds <strong>of</strong> bacteria. But the sterile samples are not the only <strong>in</strong>dication <strong>of</strong>cross-contam<strong>in</strong>ation <strong>an</strong>d the spread<strong>in</strong>g <strong>of</strong> micro-org<strong>an</strong>isms. Spread<strong>in</strong>g is als<strong>of</strong>ound among <strong>laundry</strong> items. For example, Enterobacteriaceae were foundonly on the unwashed diaper <strong>an</strong>d dishcloth, but all other items were contam<strong>in</strong>atedafter wash<strong>in</strong>g.Another <strong>in</strong>terest<strong>in</strong>g outcome <strong>of</strong> this research is the <strong>in</strong>crease <strong>in</strong> hygienic quality<strong>of</strong> the <strong>laundry</strong> items achieved by add<strong>in</strong>g <strong>an</strong> extra r<strong>in</strong>se to the wash<strong>in</strong>gprocess. This f<strong>in</strong>d<strong>in</strong>g emerged when verify<strong>in</strong>g the Sp<strong>an</strong>ish results. The Sp<strong>an</strong>ishhygiene level seems to be systematically higher th<strong>an</strong> <strong>in</strong> the other Europe<strong>an</strong>countries. One expl<strong>an</strong>ation for this could be the extra r<strong>in</strong>se, which was addedto the wash cycle due to extensive foam<strong>in</strong>g. In <strong>an</strong> additional experiment theeffect <strong>of</strong> <strong>an</strong> extra r<strong>in</strong>se was tested. The hygienic quality was improved bymore th<strong>an</strong> 1 log unit.4.6.1 Evaluation <strong>of</strong> the test methodThe repeatability <strong>an</strong>d validation <strong>of</strong> the method developed were evaluated <strong>in</strong>Chapter 4.6.1 <strong>an</strong>d 4.6.2 The results showed that the method seems to be repeatable,is valid <strong>an</strong>d reflects common daily practice. However, before br<strong>in</strong>g<strong>in</strong>gthis method to the attention <strong>of</strong> st<strong>an</strong>dardisation bodies, research <strong>in</strong>stitutes,

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