Product Manual for Dionex IonPac AS23 Columns - Cromlab
Product Manual for Dionex IonPac AS23 Columns - Cromlab
Product Manual for Dionex IonPac AS23 Columns - Cromlab
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<strong>Product</strong> <strong>Manual</strong> <strong>for</strong> <strong>IonPac</strong> <strong>AS23</strong> amd <strong>IonPac</strong> AG23 <strong>Columns</strong> Page 7 of 323.3 The Injection Loop3.3.1 The 2-mm System Injection Loop, 2 - 15 μLFor most applications on a 2-mm analytical system, a 2 - 15 μL injection loop is sufficient. Generally, you should not injectmore than 12.5 nanomoles of any one analyte onto a 2-mm analytical column. Injecting larger number of moles of a samplecan result in overloading the column which can affect the detection linearity. For low concentrations of analytes, largerinjection loops can be used to increase sensitivity. The <strong>AS23</strong> 2-mm requires a microbore HPLC system configuration. Installan injection loop one-fourth or less (