Product Manual for Dionex IonPac AS23 Columns - Cromlab

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Product Manual for IonPac AS23 amd IonPac AG23 Columns Page 7 of 323.3 The Injection Loop3.3.1 The 2-mm System Injection Loop, 2 - 15 μLFor most applications on a 2-mm analytical system, a 2 - 15 μL injection loop is sufficient. Generally, you should not injectmore than 12.5 nanomoles of any one analyte onto a 2-mm analytical column. Injecting larger number of moles of a samplecan result in overloading the column which can affect the detection linearity. For low concentrations of analytes, largerinjection loops can be used to increase sensitivity. The AS23 2-mm requires a microbore HPLC system configuration. Installan injection loop one-fourth or less (
Product Manual for IonPac AS23 amd IonPac AG23 Columns Page 8 of 323.7 Anion Atlas Electrolytic Suppressor (AAES) RequirementsAn Atlas Anion Electrolytic Suppressor (AAES) may be used instead of an ASRS ULTRA II for applications that require suppressedconductivity detection. The AAES (P/N 056116) can be used for AS23 2-mm and 4-mm applications using eluents up to 25 μeq/min.For detailed information on the operation of the Atlas Anion Electrolytic Suppressor, see Document No. 031770, the “ProductManual for the Anion Atlas Electrolytic Suppressor.”3.8 Anion MicroMembrane Suppressor (AMMS III) RequirementsAn Anion MicroMembrane Suppressor (AMMS III) may be used instead of an ASRS ULTRA II (4-mm) for applications that requiresuppressed conductivity detection. Use an AMMS III (P/N 056750) with the IonPac AS23 4-mm Analytical Column. It is compatiblewith all solvents and concentrations with which the systems and columns are compatible. For 2-mm operation, use the AMMS III(P/N 056751).For detailed information on the operation of the Anion MicroMembrane Suppressor, see Document No. 031727, the “Product Manualfor the Anion MicroMembrane Suppressor III, the AMMS III.”3.9 Using AutoRegen with the ASRS ULTRA II or the AMMS III in the Chemical SuppressionModeTo save regenerant preparation time and reduce regenerant consumption and waste, Dionex recommends using an AutoRegen ®Accessory (P/N 039594). For more detailed information on the use of the AutoRegen Accessory see the AutoRegen Accessorymanual (Document No. 032853). For more detailed information on the use of AutoRegen Regenerant Cartridges, see the “ProductManual for the AutoRegen Regenerant Cartridge Refills” (Document No. 032852).3.10 Using Displacement Chemical Regeneration (DCR) with the Chemical Suppression ModeDionex recommends using the Displacement Chemical Regeneration (DCR) Mode for chemical suppression using sulfuric acid(H 2SO 4) and the Anion MicroMembrane Suppressor (AMMS III). See the DCR kit manual, Document P/N 031664, for details.SAFETYUse proper safety precautions in handling acids and bases.3.11 Detector RequirementsSee Appendix B, “Comparison of 2-mm and 4-mm Ion Chromatography Systems,” for 2-mm and 4-mm system detector, cell andthermal stabilizer requirements.Document No. 065120-05 ©2012 Thermo Fisher Scientific July 2012
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Product Manual for Dionex IonPac AS23 Columns - Cromlab

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