Product Manual for Dionex IonPac AS23 Columns - Cromlab

Product Manual for IonPac AS23 amd IonPac AG23 Columns Page 11 of 324.4 Making Eluents that Contain SolventsWhen mixing solvents with water remember to mix solvent with water on a volume to volume basis. If a procedure requires aneluent of 90% acetonitrile, prepare the eluent by adding 900 mL of acetonitrile to an eluent reservoir. Then add 100 mL ofdeionized water or eluent concentrate to the acetonitrile in the reservoir. Using this procedure to mix solvents with water willensure that a consistent true volume/volume eluent is obtained. Premixing water with solvent will minimize the possibility ofoutgassing.NOTENOTENOTEWhen purging or degassing eluents containing solvents, do not purge or degas the eluent excessivelysince it is possible that a volatile solvent can be “boiled” off from the solution.Always degas and store all eluents in glass or plastic eluent bottles pressurized with helium. Onlyhelium can be used to purge and degas ionic eluents containing solvents, since nitrogen is soluble insolvent containing eluents.Acetonitrile (ACN) hydrolyzes to ammonia and acetate when left exposed to basic solutions. To preventeluent contamination from acetonitrile hydrolysis, always add acetonitrile to basic aqueous eluents byproportioning the acetonitrile into the basic eluent with the gradient pump. Keep the acetonitrile in aseparate eluent bottle containing only acetonitrile and water.SAFETYNEVER ADD THE ACETONITRILE DIRECTLY TO THE BASIC CARBONATE OR HYDROXIDE ELUENTSOLUTIONS.4.5 Regenerant Preparation for the AMMS IIIThe Anion MicroMembrane Suppressor III (AMMS III) requires the use of a regenerant solution. If you are using the AMMS IIIinstead of the Anion Self-Regenerating Suppressor ULTRA II (ASRS ULTRA II) see Document No. 031727, the “Product Manualfor the Anion MicroMembrane Suppressor III, the AMMS III.”Document No. 065120-05 ©2012 Thermo Fisher Scientific July 2012