An Introduction to qRT-PCR Assays Using Sybr Green (Using SDS ...

efficiency will give a scale of 1 (no change in product per cycle) to 2 (perfectdoubling). The two are interchangeable – simply add or subtract 1.Kinetic ApproachesRather than use artificial standards, amplification efficiency can bedetermined from the kinetics of the amplification plots produced by everysample. As such, the efficiency of every sample may be determined with noadditional samples required.We have automated this procedure in a freely-available Excelworkbook named DART-PCR, and found it to give comparable data toexisting methods. An example data set described in the paper is also available(Peirson et al., 2003). Basically, this workbook performs all the necessarycalculations – those interested are directed to the paper for a furtherexplanation!