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ACADEMIA SINICA3 CaM/SiNW-FETTnISiNW-FETp-a100M Ca 2+ 0.1PSTnI231nMCaM/SiNW-FETbCaM/SiNW-FET231nM100M Ca 2+ 0.1PSTnI231nM100M Ca 2+ 0.1PSTnI231nM500M EDTAethylenediaminetetraaceticacid0.1PScCaM/SiNW-FETGlog[TnI]TnI100M Ca 2+ 0.1PS500M EDTA0.1PS0.987d10 -4 M Ba 2+ 10 -8 M Ca 2+ 10 -7M Ca 2+ 10 -6 M Ca 2+ TnI231nMCaM/SiNW-FET 肽 glutathioneGSH 肽 酶 glutathione S-transferaseFET2GSHSiNW-FETGSTSiNW-GSH/SiNW-FETGSTCaMCaM-GSTGSTGSH/SiNW-FETCaM/SiNW-FETCaM/SiNW-FETCaMGSH≥1mMCaM-GSTSiNW-FETGSHCaMTnI3a100M Ca 2+ 塩 0.1phosphate solution0.1PS240M NaH 2 PO 4 760M Na 2 HPO 4 pH7.4NaOHTnIpI4 SiNW-FETp-CaMN-VGCCaN-VGCCPDMSCaM/SiNW-FETSiNW-FETb–cpH7.4N-VGCCpI=8.79IQCaMCa 2+ b100M Ca 2+ c500MEDTASiNW-FETd1bN-VGCCCaMN-VGCCCaMSiNW-FETN-VGCCSiNW-FETCaMN-VGCC9.3CaM/SiNW-FETgatingeffectp-SiNW-FET3bCaM/SiNW-FETTnICa 2+TnITnICaMcGSiNW-FETlog[TnI]K a 10 7 M -1 Ca 2+ 3bCaMTnICa 2+ [Ca 2+ ]CaM-TnI[Ca 2+ ]=10 -8 –10 -6 MTnICaM/SiNW-FET3d[Ca 2+ ]=1M≥3CaM-TnICaMN-VGCC4N-VGCCN-VGCCCaM/SiNW-FETSiNW-FETCaMN-VGCCSiNW-FET“Label-free Detection of Protein-protein Interactions Using aCalmodulin-modified Nanowire Transistor”, Proceedings of theNational Academy of Sciences of the U. S. A. 107 (2010): 1047-1052.25
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