Document - FAVV

4.1.4.2 Work package II: Cross reactivity ............................................................................................................................................................ 4-414.1.4.2.1 Cross reactivity in solvent ................................................................................................................................................................... 4-414.1.4.2.1.1 Study design ............................................................................................................................................................................. 4-414.1.4.2.1.2 Data handling and statistical analyses ........................................................................................................................................ 4-414.1.4.2.1.2.1 Elisa kits .................................................................................................................................................................................................... 4-414.1.4.2.1.2.2 LFD............................................................................................................................................................................................................ 4-414.1.4.2.2 Cross reactivity in matrix ................................................................................................................................................................... 4-414.1.4.2.2.1 Study design ............................................................................................................................................................................. 4-414.1.4.2.2.2 Data handling and statistical analyses ........................................................................................................................................ 4-424.1.4.3 Work package III: Matrix effect .............................................................................................................................................................. 4-424.1.4.3.1 Study design ...................................................................................................................................................................................... 4-424.1.4.3.2 Data handling and statistical analyses ................................................................................................................................................ 4-424.1.4.3.2.1.1 Elisa kits .................................................................................................................................................................................................... 4-424.1.4.3.2.1.2 LFD............................................................................................................................................................................................................ 4-444.1.4.4 Work package IV: Accuracy ................................................................................................................................................................... 4-444.1.4.4.1 Study design ...................................................................................................................................................................................... 4-444.1.4.4.2 Data handling and statistical analyses ................................................................................................................................................ 4-444.1.4.5 Work package V: Blank sample analysis................................................................................................................................................. 4-444.1.4.5.1 Study design ...................................................................................................................................................................................... 4-444.1.4.5.2 Data handling and statistical analyses ................................................................................................................................................ 4-444.1.5 Extraction protocols .................................................................................................................................................................................... 4-454.2 ROSA OCHRATOXIN GRAIN by CHARM ............................................................................................................ 4-464.2.1 Concentration accuracy of standards......................................................................................................................................................... 4-464.2.2 Cross-reactivity ............................................................................................................................................................................................. 4-464.2.2.1 Solvent .................................................................................................................................................................................................... 4-464.2.2.2 Matrix ................................................................................................................................................................................................... 4-474.2.3 Matrix effect ................................................................................................................................................................................................. 4-484.2.4 Accuracy ....................................................................................................................................................................................................... 4-494.2.5 Blank sample analysis .................................................................................................................................................................................. 4-494.2.6 Conclusions .................................................................................................................................................................................................. 4-504.3 RIDASCREEN® Ochratoxin A 30/15 by R-Biopharm ............................................................................................ 4-514.3.1 Concentration accuracy of standards......................................................................................................................................................... 4-514.3.2 Cross-reactivity ............................................................................................................................................................................................. 4-514.3.2.1 Solvent .................................................................................................................................................................................................... 4-514.3.2.2 Matrix ................................................................................................................................................................................................... 4-524.3.3 Matrix effect ................................................................................................................................................................................................. 4-534.3.4 Accuracy ....................................................................................................................................................................................................... 4-534.3.5 Blank sample analysis .................................................................................................................................................................................. 4-544.3.6 Conclusions .................................................................................................................................................................................................. 4-544.4 AgraQuant® Ochratoxin by Romerlabs ........................................................................................................................ 4-554.4.1 Concentration accuracy of standards......................................................................................................................................................... 4-554.4.2 Cross-reactivity ............................................................................................................................................................................................. 4-554.4.2.1 Solvent .................................................................................................................................................................................................... 4-554.4.2.2 Matrix ................................................................................................................................................................................................... 4-564.4.3 Matrix effect ................................................................................................................................................................................................. 4-574.4.4 Accuracy ....................................................................................................................................................................................................... 4-574.4.5 Blank sample analysis .................................................................................................................................................................................. 4-584.4.6 Conclusions .................................................................................................................................................................................................. 4-584.5 OCHRATOXIN-A ELISA by Europroxima ............................................................................................................... 4-594.5.1 Concentration accuracy of standards......................................................................................................................................................... 4-594.5.2 Cross-reactivity ............................................................................................................................................................................................. 4-594.5.2.1 Solvent .................................................................................................................................................................................................... 4-594.5.2.2 Matrix ................................................................................................................................................................................................... 4-604.5.3 Matrix effect ................................................................................................................................................................................................. 4-604.5.4 Accuracy ....................................................................................................................................................................................................... 4-614.5.5 Blank sample analysis .................................................................................................................................................................................. 4-624.5.6 Conclusions .................................................................................................................................................................................................. 4-624.6 Conclusions of the experimental evaluation .................................................................................................................. 4-635. Conclusion and future prospects ............................................................................... 5-65