Document - FAVV
Document - FAVV
Document - FAVV
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Evaluation of immunoassay kits for determination of ochratoxin A in cereals3.3 Conclusions of the administrative evaluationThe performance of ELISA and LFD kits for OTA was compared for the following parameters: concentrationrange, applicable matrices, limit of detection, limit of quantification, recovery, accuracy, precision, repeatability,reproducibility, measurement uncertainty and cross-reactivity. For qualitative kits, false negative/false positive rates,accuracy and cross-reactivity were compared.From this exercise it is clear that the kits fulfil the requirements to be used as fast screening tools. In terms ofpurchasing cost, material and consumable requirements and labour cost they are competitive compared to thereference methods. Results are in general biased slightly positively leading to an increase in false positive rate. Assamples labelled as positive are to be screened by a significantly more expensive reference method, a too high falsepositive result rate will compromise the cost efficiency.CODA-CERVA | Administrative evaluation 3-35