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Evaluation of immunoassay kits for determination of ochratoxin A in cerealsSpecific criteria for relative accuracy, relative sensitivity and relative specificity are not set by regulatory organs, butthe data for the Europroxima kit (Table 36) indicate no specific problems.3.2.4.5 Cross-reactivityTable 37: Cross-reactivity in%Manufacturer OTA OTB OTC Otα Cou 4-HC CIT PhA ZEA AFLA B1EuroProxima BV 100 9 NS NS NS NS NS NS NS NSR-Biopharm AG 100 NS NS
Evaluation of immunoassay kits for determination of ochratoxin A in cereals3.3 Conclusions of the administrative evaluationThe performance of ELISA and LFD kits for OTA was compared for the following parameters: concentrationrange, applicable matrices, limit of detection, limit of quantification, recovery, accuracy, precision, repeatability,reproducibility, measurement uncertainty and cross-reactivity. For qualitative kits, false negative/false positive rates,accuracy and cross-reactivity were compared.From this exercise it is clear that the kits fulfil the requirements to be used as fast screening tools. In terms ofpurchasing cost, material and consumable requirements and labour cost they are competitive compared to thereference methods. Results are in general biased slightly positively leading to an increase in false positive rate. Assamples labelled as positive are to be screened by a significantly more expensive reference method, a too high falsepositive result rate will compromise the cost efficiency.CODA-CERVA | Administrative evaluation 3-35
Page 1: CODA-CERVAEvaluation of immunoassay
Page 5: We would like to thank the kit manu
Page 8 and 9: 4.1.4.2 Work package II: Cross reac
Page 10 and 11: Table 45: Cross-reactivity in matri
Page 13: 1. Foreword & scope &background
Page 16 and 17: Evaluation of immunoassay kits for
Page 21: Evaluation of immunoassay kits for
Page 27: 3. Administrative evaluation
Page 37 and 38: Measured value (ng/g)Evaluation of
Page 51: 4. Experimental evaluation
Page 60 and 61: ()Evaluation of immunoassay kits fo
Page 62 and 63: Observed Concentration (ng/g)Observ
Page 79: 5. Conclusion and futureprospects

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