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Measured value (ng/g)Evaluation of immunoassay kits for determination of ochratoxin A in cerealsDifference % (X e -X t )/X t50%40%30%20%10%0%-10%-20%-30%-40%-50%0 2 4 6 8 10 12 14 16Reference Value (ng/g)Figure 6: Descriptive comparison of data in relative values obtained via R-Biopharm3.1.4.5.2.2.2 Ordinary linear regressionThe results of the linear regression are summarised inFigure 7 and Table 20. ρ c indicates excellent agreement.1816141210864200 2 4 6 8 10 12 14 16Reference value (ng/g)Figure 7: Linear fit of measured OTA concentration versus reference value for R-Biopharm3-24 Administrative evaluation | CODA-CERVA
Evaluation of immunoassay kits for determination of ochratoxin A in cerealsTable 20: Summary of the ordinary linear regression for R-BiopharmCoefficientsSlope 1.119Intercept -0.580R 2 0.958ρc 0.969T-Test 0.8803.1.4.5.2.2.3 ConclusionAn overall good agreement is seen between results obtained by R-Biopharm and the reference value. OTA immunoassa-OTA reference is not statistically dependant on OTA levels.3.1.4.6 Intra-run repeatability, inter-run repeatability and reproducibilityAccording to directive 401/2006 ref10 the relative standard deviation, calculated from results generated underrepeatability conditions should be ≤ 20% (1-10 ng/g), relative standard deviation calculated from results generatedunder reproducibility conditions should be ≤ 30% (1-10 ng/g).3.1.4.6.1 Intra-run repeatability (same batch/operator)The extended measurement uncertainty is in general well below 40%, (Table 21) indicating results are reproducible.For the Charm kit it can be seen that results vary significantly at low concentration, at high concentration the kitgenerates very reproducible results.CODA-CERVA | Administrative evaluation 3-25
Page 1: CODA-CERVAEvaluation of immunoassay
Page 5: We would like to thank the kit manu
Page 8 and 9: 4.1.4.2 Work package II: Cross reac
Page 10 and 11: Table 45: Cross-reactivity in matri
Page 13: 1. Foreword & scope &background
Page 16 and 17: Evaluation of immunoassay kits for
Page 21: Evaluation of immunoassay kits for
Page 27: 3. Administrative evaluation
Page 37: Measured value (ng/g)Evaluation of
Page 51: 4. Experimental evaluation
Page 60 and 61: ()Evaluation of immunoassay kits fo
Page 62 and 63: Observed Concentration (ng/g)Observ
Page 79: 5. Conclusion and futureprospects

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