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Evaluation of immunoassay kits for determination of ochratoxin A in cereals3.1.4.5.2.1 Charm Science3.1.4.5.2.1.1 Descriptive comparisonFigure 4 summarizes the difference in relative values. When looking at the results it seems that at the legal limitsamples tend to bias a little high, at high concentrations they bias a little bit low.50%40%30%20%Difference % (X e -X t )/X t10%0%-10%-20%-30%-40%-50%0 10 20 30 40 50 60 70 80 90 100 110 120Reference Value (ng/g)Figure 4: Descriptive comparison of data in relative values obtained via Charm3.1.4.5.2.1.2 Ordinary linear regressionConcordance between the LFD and the reference values was checked by linear regression, results are summarised inFigure 5 and Table 19. ρ c indicates overall good agreement.3-22 Administrative evaluation | CODA-CERVA
Measured value (ng/g)Evaluation of immunoassay kits for determination of ochratoxin A in cereals10090807060504030201000 20 40 60 80 100 120Reference value (ng/g)Figure 5: Linear fit of measured OTA concentration versus reference value for CharmTable 19: Summary of the ordinary linear regression for CharmCoefficientsSlope 0.783Intercept 1.837R 2 0.988ρc 0.960T-test 0.7503.1.4.5.2.1.3 ConclusionAn overall good agreement between the reference values is obtained, only at low or high concentrations there issome deviation.3.1.4.5.2.2 R-Biopharm3.1.4.5.2.2.1 Descriptive comparisonThe results (Figure 6) shows random scattering around the ordinate.CODA-CERVA | Administrative evaluation 3-23
Page 1: CODA-CERVAEvaluation of immunoassay
Page 5: We would like to thank the kit manu
Page 8 and 9: 4.1.4.2 Work package II: Cross reac
Page 10 and 11: Table 45: Cross-reactivity in matri
Page 13: 1. Foreword & scope &background
Page 16 and 17: Evaluation of immunoassay kits for
Page 21: Evaluation of immunoassay kits for
Page 27: 3. Administrative evaluation
Page 35: Evaluation of immunoassay kits for
Page 51: 4. Experimental evaluation
Page 60 and 61: ()Evaluation of immunoassay kits fo
Page 62 and 63: Observed Concentration (ng/g)Observ
Page 79: 5. Conclusion and futureprospects

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