Document - FAVV

Evaluation of immunoassay kits for determination of ochratoxin A in cereals3.1.4.5.1 Accuracy and measurement uncertaintiesTable 18: Accuracy and measurement uncertaintiesCharm SciencesMatrixTrue Measured(ng/g) (ng/g)Rec% N CV% Ucomb k=2 (%)Wheat 0 0.1 / 21 401 0.8 80.0 21 50 107.75 6 120.0 21 13.33 48.1Barley 0 0.1 / 21 203.5 4.9 140.0 21 10.2 82.620 21.4 107.0 21 10.28 24.9EuroProxima BVMatrixTrue Measured(ng/g) (ng/g)Rec% N CV% Ucomb k=2 (%)Green Coffee 7.53 7.7 102.3 8 2.3 6.4(FAPAS) 7.67 8.4 109.5 8 9.5 26.96.24 7 112.2 8 12.2 34.5Barley 3.52 3.6 102.3 8 2.3 6.5(FAPAS)Wine 1.02 1 98.0 8 2 5.6(FAPAS) 1.63 1.24 76.1 8 24 67.8LCTech GmbHMatrixTrue Measured(ng/g) (ng/g)Rec% N CV% Ucomb k=2 (%)Roasted Coffee 6.1 5.5 90.2 4 6 23.0(ERM)Green Coffee 9.17 7.4 80.7 4 11.4 44.8(FAPAS)Instant Coffee 14.9 15.3 102.7 4 10.2 21.1(FAPAS)Coffee 15.2 18.6 122.4 3 21.6 62.2R-BiopharmMatrixTrue Measured(ng/g) (ng/g)Rec% N CV% Ucomb k=2 (%)Wheat 2 2.5 125.0 3 7 51.95 5.2 104.0 3 13.9 28.97.4 6.7 90.5 3 10.1 27.7RomerlabsMatrixTrue Measured(ng/g) (ng/g)Rec% N CV% Ucomb k=2 (%)Corn 5 4.59 91.8 90 12.8 30.410 8.53 85.3 90 8.2 33.720 17.02 85.1 90 9 34.830 25.97 86.6 90 10.3 33.9Accuracies are well within the mandatory range, only Charm for barley and LCTech for coffee bias somewhat.Expanded measurement uncertainty is in general lower than 50%, Charm is somewhat higher at 1 ng/g.3.1.4.5.2 Confirmation (ELISA/LFD versus reference values)For the two kit producers that provided extensive information on accuracy for cereals, the data were assessed usingthe following methodology;Descriptive comparison of data obtained via ELISA/LFDOrdinary linear regression with Lin‟s concordance coefficientCODA-CERVA | Administrative evaluation 3-21