Document - FAVV

Evaluation of immunoassay kits for determination of ochratoxin A in cerealsTable 16: Calculation of LOD and LOQAokin AG Charm EuroProxima LCTech R-Biopharm RomerlabsLOD AOAC ν ν νOther Matrix + 2SD Matrix + 2SDLOQ AOAC ν ν νOther Matrix + 10SD Matrix + 3SDUnlike for DON 64 , no explicit LOD requirements are set for OTA. Evidently the LOD, and preferably also theLOQ, should be lower than the legal limits set for a certain matrix if applicable. For wheat in the EU, the limit is setat 5 ng/g for unprocessed wheat and 3 ng/g 3 for flour. From Table 15 it can be seen that all kits are compliant.3.1.4.4 RecoveryEU directives 10 state that recovery should be between 70 and 110% for the concentration range 1-10 ng/g. Most kitsare compliant with the limits set for OTA recovery. The error range is in general higher for lower concentrations,which is to be expected 65 66 .Table 17: RecoveryAokinMatrix ng/g Rec% N CV% Ucomb k=2 (%)Common 2 85 3 8.2 34.2Matrix 5 89 3 11.2 31.410 79 3 11.4 47.8Charm SciencesMatrix ng/g Rec% N CV% Ucomb k=2 (%)Wheat 1 80 21 50 107.75 120 21 13.3 48.010 116 21 6 34.2EuroProximaMatrix ng/g Rec% N CV% Ucomb k=2 (%)Cereal 2.5 95 6 6.7 16.75 90 6 8.3 26.07 73 6 4.7 54.8Green 2.5 74 6 4 52.6Coffee 5 97 6 3 8.57 114 6 4 29.1Beer 2.5 79 20 5 43.25 86 6 4 29.17 93 6 3 15.2Chocolat 2.5 115 8 5 31.65 82 6 5 37.47 101 6 3 6.364http://www.gipsa.usda.gov/GIPSA/webapp?area=home&subject=lr&topic=hb-don65W. Horwitz, L.R. Kamps and K.W. Boyer, J. Assoc. Off. Anal. Chem., 63, 1344 (1980)66W. Horwitz, J. Assoc. Off. Anal. Chem., 86, 109 (2003CODA-CERVA | Administrative evaluation 3-19