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Edexcel Level 3 BTEC Nationals in Health and Social Care

Edexcel Level 3 BTEC Nationals in Health and Social Care

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UNIT 16: SCIENCE IN PRACTICE FOR HEALTH3 Be able to use microscopic techniquesPreparation of slides: fix<strong>in</strong>g, sta<strong>in</strong><strong>in</strong>g techniquesUse of light microscope: <strong>in</strong> exam<strong>in</strong>ation of prepared slides, differentmagnifications, <strong>in</strong>terpretationMicroscopes: light, UV, electron, SEM, TEM; images, limitations, applications4 Be able to use aseptic techniques to culture microorganismsMicroorganisms: eg from yoghurtTechniques: laboratory aseptic techniques; sterile techniques for collection ofswabs; pr<strong>in</strong>ciples of media cultures, preparation, <strong>in</strong>oculation, <strong>in</strong>cubation <strong>and</strong>microbiological transfer; count<strong>in</strong>g techniques, factors affect<strong>in</strong>g growth ofmicroorganisms; sta<strong>in</strong> tests for diagnosis, eg gram sta<strong>in</strong>; antibiotic sensitivity<strong>Health</strong> <strong>and</strong> safety: codes of practice for microbiology laboratories — compliancewith COSHH regulationsInvestigation: eg effectiveness of different antiseptics/dis<strong>in</strong>fectants, lowesteffective concentration of antiseptics/dis<strong>in</strong>fectants, growth requirements ofparticular bacteria, effect of length of exposure to UV light, changes <strong>in</strong> thebacterial count <strong>in</strong> stored yoghurt; use of aseptic technique, culture techniques,sta<strong>in</strong><strong>in</strong>g <strong>and</strong> observ<strong>in</strong>g microbial cultures; health <strong>and</strong> safety as appropriate totasksBN018467 – Specification – <strong>Edexcel</strong> <strong>Level</strong> 3 <strong>BTEC</strong> <strong>Nationals</strong> <strong>in</strong> <strong>Health</strong> <strong>and</strong> <strong>Social</strong> <strong>Care</strong>– Issue 1 – February 2007 © <strong>Edexcel</strong> Limited 2007219

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