Curriculum Vitae - Royan Institute

Curriculum Vitae1) Personal Informaon:Surname: TAVALAEEFirst name: MARZIYEHGender: FemaleNationality: IranianDate of birth: year: 1978 month:7 day: 28Place of Birth: EsfahanMarital Status: unmarriedOccupation: M.ScHead of Andrology Department of Royan Institute (Isfahan campus)Address: Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranP.O. Box: 19395-4644, Tehran, IRAN.TEL: 0098 311 2612900-3FAX: 0098 3112605525E-MAIL: Tavalaee.m@gmail.com ; tavalaee.royan@gmail.com

ActivitiesInternational ArticlesNational Articles (ISI)National Research & Scientific ArticlesConferences and MeetingsProjectsPrizePresentation of WorkshopConsulting of thesisBook publications:Number1371313173342

2) Educaonal background:Degree Major University DateB.ScM.ScBiologyAnimal SciencesBiologyFalavarjan Azad Uni.(Esfahan, Iran) 1997-2001Damghan Azad Uni.(Damghan, Iran) 2004-2006

7- Kheirollahi-Kouhestani M, Razavi S, Tavalaee M, Deemeh MR, Mardani M, Moshtaghian J, Nasr-Esfahani MH.Selection of sperm based oncombined density gradient and Zeta method may improve ICSI outcome.Hum Reprod. 2009 Oct;24(10):2409-16. Epub 2009 Jun 24.8- Nasr-Esfahani MH, Deemeh MR, Tavalaee M. Artificial oocyte activation and intracytoplasmic sperm injection. Ferl Steril. 2009 Apr 24.9- Tavalaee M, Razavi S, Nasr-Esfahani MH. Influence of sperm chromatin anomalies on assisted reproductive technology outcome. FertilSteril. 2009 Apr;91(4):1119-26. Epub 2008 May 27.10- Nasr-Esfahani MH, Razavi S, Vahdati AA, Fathi F, Tavalaee M.Evaluation of sperm selection procedure based on hyaluronic acid bindingability on ICSI outcome.J Assist Reprod Genet. 2008 May;25(5):197-203. Epub 2008 May 16.11- Nasr-Esfahani MH, Razavi S, Javdan Z, Tavalaee M.Artificial oocyte activation in severe teratozoospermia undergoing intracytoplasmicsperm injection.Ferl Steril. 2008 Dec;90(6):2231-7. Epub 2008 Jan 18.12- Nasr-Esfahani MH, Abasi H, Razavi S, Ashrafi S, Tavalaee M.Varicocelectomy: semen parameters and protamine deficiency. Int J Androl.2009 Apr;32(2):115-22. Epub 2007 Oct 30.13- Nasr-Esfahani MH, Razavi S, Tavalaee M.Failed fertilization after ICSI and spermiogenic defects.Ferl Steril. 2008 Apr;89(4):892-8. Epub2007 Jun 21.

4) Articles published in National Journal s (ISI): 71- Tavalaee , Razavi, Nasr-Esfahani. Effect of sperm acrosomal integrity and protamine deficiency on IVF and pregnancy rate. Iranian fertilityand sterility journal. Spring 2007, 1; 27-34.2- Deemeh,Tavalaee, Razavi, Nasr-Esfahani. Evaluation of protamine deficiency and DNA fragmentation in two globozoospermia patientsundergoing ICSI. Iranian ferlity and sterility journal, Summery 2007, 1(2); 85-88.3- Salamian, Ghaedi, Razavi, Tavalaee, Tanhaei, Tavalaee, salahshouri, Gourabi, Nasr-Esfahani. Single Nucleotide Polymorphism Analysis ofProtamine Genes in Infertile Men. International Journal of Fertility and Sterility.May-Jun 2008, 2(1); 13-18.4- Khajavi, RazaviMardani, Tavalaee, Deemeh, , Nasr-Esfahani, Can Zeta sperm selection method, recover sperm with higher DNA integritycompare to density gradient centrifugation? Iranian Journal of Reproducve Medicine. Spring 2009, 7(2); 73-77.5- Tavalaee, Kiani, Arbabian, Deemeh, Nasr-Esfahani. Flow Cytometry: A New Approach for Indirect Assessment of Sperm ProtamineDeficiency. Internaonal Journal of Ferlity and Sterility. Vol 3, No 4, Feb-Mar 2010, Pages: 177-184.6- Deemeh, Tavalaee, Ahamadi, Kalantari, Alavi Nasab, Najafi,Nasr Esfahani. The First Report of Successfully Pregnancy After ICSI withCombined DGC/Zeta Sperm Selection Procedure in a Couple with Eleven Repeated Fail IVF/ICSI Cycles. . International Journal of Fertility andSterility. Vol 4, No 1,2010.

7- Nasr Esfahani, Abbasi, Mirhosseini, Ghasemi, Razavi, Tavalaee, Tanhaei, Deemeh, Ghaedi, Zamansoltani, Rajaei. Can Altered Expression ofHSPA2 in Varicocele Paents Lead to Abnormal Spermatogenesis? International Journal of Fertility and Sterility. Vol 4, No 3, Oct-Dec 2010,5) Articles published in National Research & Scientific Journals: 131- Taghadosi, Tavalaee, Razavi, Mardani, Nasr Esfahani, Efficiency of Sperm Gradient Processing and HA-binding Test for the Selection ofSperm with Normal Protamine, intact DNA and Normal Morphology. Yakhteh Medical Journal, 2008; 9(4):226-231.2- Razavi, Nasr-Esfahani, Tavalaee, Ameri. Assessing the sperm DNA damages on Assisted Reproductive Technology outcome. Journal of ArakUniversity of Medical Sciences. 2008;11(1):21-31.3- Fathi, Razavi, Vahdati, Nasr-Esfahani, Tavalaee. Evaluation of sperm selection procedure based on its binding ability to hyaluronic acide onICSI outcome. Journal of Isfahan Medical School. 2008; 26(89): 187-197.4- Nasr-Esfahani, Tavalaee, Deemeh. Origins and Evaluation of DNA Damage in Infertile Individual. Journal of Iranian Anatomical Sciences.2008;6(24): 489-50.5- Fathi, Vahdati, Nasr-Esfahani, Razavi, Tavalaee. Relation between sperm binding ability to hyaluronic acid with human sperm parameters.Iranian biology Journal. 2008;21(5):1-8.6- Nasr-Esfahani, Razavi, Mardani, Khajavi, Deemeh, Tavalaee. The Comparison of Efficiency of Density Gradient Centrifugation and ZetaMethods in Separaon of Mature Sperm with Normal Chroman Structure. Yakhteh Medical Journal, 2009;11(2):168-175.7- Deemeh, Nasr-Esfahani, Razavi Nazem, Shayeste Moghadam, Tavalaee. The comparison of HA binding and Zeta methods efficiency inselection of sperm with normal morphology and intact chromatin. Journal of Isfahan Medical School, 2009; 27(92):46-56.8- Shayesteh Moghadam, Nasr-Esfahani, Razavi,Nazem,Deemeh, Tavalaee .The efficiency of zeta method in separation of sperm with normalmorphology and chromatin structure.Journal of Shahrekord University of Medical Sciences, 2008;10(1):20-29.

9- Razavi, Nasr-Esfahani, Tavalaee, vaezi, Shiravi. Relationship between protamine deficiency and acrosin activity with fertilization rate postICSI. Journal of Iranian Anatomical Sciences, 2006; 3(4):245-252.10- Ashrafi, Nasr-Esfahani, Razavi, Abasi, Molavi, Tavalaee. Effect of varicocelectomy on semen parameters and protamine deciency 3 and 6months post surgery. Journal of Isfahan Medical School, 2007; 25, 87.11- Rezaei, Fathi, Tavalaee,Vahdati, Nasr Esfahani. Relation between sperm binding ability to hyaluronic acid with protamine deficiency andDNA fragmentaon. Journal of Iranian Anatomical Sciences. 2007; 5(18):49-59.12- Nasr Esfahani, Rezaei, vaezi, shiravi, Tavalaee. Effects of acrosomal activity and morphology on fertilization rates following ICSI. Journal ofreproduction and infertility. 2006; 7(3): 28.13- Tavalaee, Razavi, Nasr-Esfahani. Effects of Spermiogenic Defects on Fertilization and Pregnancy Rate in IVF Patients. Yakhteh MedicalJournal, Summer 2007, 9(2).

6) Conferences and Meetings:No TITLE AUTHOR LOCATION PRES. DATE1 Evaluation sperm DNA damage and its influenceon infertilityTavalaee 11th Congress on Royan Internaonal Oral 20102Influence of sperm chromatin anomalies onART outcomeTavalaee9 th Congress on Reproductive Biomedicine4th Congress on Stem Cell Biology &TechnologyOral 20083The comparison of density gradiantcentrifugation and Zeta methods inseparation of mature and normalspermatozoa by TUNEL, SCD, AO and CMA3Khajavi, Razavi,Tavalaee, Deemeh, Nasr-Esfahani9 th Congress on Reproducve Biomedicine4th Congress on Stem Cell Biology &TechnologyOral 20084Relationship between protamine deficiencyand acrosomal structure with pregnancy ratepost IVFTavalaee, Razavi, Nasr-Esfahani13 th Congress Of Iranian society forreproductive medicine Poster 20075Sperm selection with normal morphologyand chromatin using HA-bindingTaghadosi, Nasr-Esfahani, Razavi,Mardani, Tavalaee13 th Congress Of Iranian society forreproductive medicineOral 20076 Effect of Ionomycin as oocyte activationfactor in oligoasthenoteratozoospermicRazavi, Javdan, Nasr-Esfahani, Tavalaee13 th Congress Of Iranian society forreproductive medicine Oral 2007

patients post ICSI7Relationship between protamine deficiencyand acrosin activity with fertilization ratepost ICSI.Tavalaee, Razavi, Nasr-Esfahani13 th Congress Of Iranian society forreproductive medicine Oral 20078Effect of varicocelectomy on protaminedeciency 3 and 6 months post surgery.Ashrafi, Nasr-Esfahani,Razavi, Tavalaee13 th Congress Of Iranian society forreproductive medicine Oral 20079Relationship between banded sperm to acidhyaluronic with DNA fragmentation andsperm parameters.Fathi, Nasr-Esfahani,Razavi, Vahdati,Tavalaee13 th Congress Of Iranian society forreproductive medicineOral 200711Comparison of sperm parameters before andafter varicocelectomy and its effect onfertility.Ashrafi, Nasr-Esfahani,Razavi, Tavalaee13 th Congress Of Iranian society forreproductive medicine Oral 200712Effects of acrosomal activity and morphologyon fertilization rates following ICSINasr Esfahani, Rezaei,Tavalaee.vaezi, shiravi2 th iranbioconference Poster 200613Relationship between protamine deficiencyand acrosin activity with fertilization ratepost ICSIRazavi, Nasr-Esfahani,Tavalaee,vaezi, Shiravi 2 th iranbioconference Poster 2006

12 SNP analyses of protamine genes ininfertile men population.Salamyian, Nasr-Isfahani,Ghaedi, Tavallayie,Razavi, Tanhaei,Tavallayie10th Iranian Genecs Congress,Tehran, IranPoster20087) Projects: 17No TITLE Director/colleagueDATEGrant1Effect of Ionomycin as oocyte activation factor inoligoasthenoteratozoospermic patients post ICSIcolleagueSummery2006Royan Institute & EsfahanUniversity of Medical Sciences2 Study of relationship between protamin deficiency with acrosin activity,sperm normal morphology and fertilization rate in candidat of ICSIinfertile couplescolleagueWinter2006Royan Institute & Damghan azadUniversity3 The study of relationship between epigenetic changes and semenparameters, DNA fragmentation, protamine deficiency in ICSI and IVFpatientsDirectorSpring2006 Royan Institute4 The effect of varicocelectomy on recovery of sperm morphology and colleague Summer Royan Institute & Payame Noor

protamine deciency at 3 and 6 months post surgery. 2007 University5 Sperm selection based on its binding ability to hyaluronic acid for intracytoplasmic sperm injection.colleagueSummery2007Royan Institute & IsfahanUniversity6 Evaluation of the relationship Between protamine deficiency and hyaluronicacid binding capacity of sperm.colleagueSpring2007Royan Institute & EsfahanUniversity of Medical Sciences7 The study of relationship between lymphocyte and sperm DNAfragmentation in infertile patient.colleagueAutumn2008Royan Institute & EsfahanUniversity of Medical Sciences8 Comparison between HA binding ability and Zeta method to select maturespermatozoa.colleagueSpring2008Royan Institute& Payame NoorUniversity9 Evaluation of leptin receptor expression in fertile and infertile mencolleagueWinter2008Royan Institute &University of science &Culture10 The comparison of efficiency density gradiant centrifugation and Zetamethods in separation of mature sperm with normal Protamine and DNAby flow cytometerycolleagueWinter2008Royan Institute & EsfahanUniversity of Medical Sciences

11 Evaluaon of HSPA2 gene expression level and it's correlaon with DNAfragmentation and protamine deficiency in varicocele patients in pre- andpost varicocelectomy.colleagueWinter2008Royan Institute &University of science &Culture12 SNP Analysis of Protamine Genes in Infertile Men Population. colleague Spring2008Royan Institute &Imam Hossein University13 The study of efficiency of Zeta method in separation of sperm With normalmorphology and chromatin structure.colleagueSpring2008Royan Institute& Payame NoorUniversity14 Comparison of sperm morphology, DNA fragmentation, protaminedeciency and HSPA2 expression before, three and six month aftercolleagueWinter2009Royan Institute & QazvinUniversity of Medical Sciencesvaricocelectomy.15 The comparison of efficiency density gradiant centrifugation and Zetamethods on ICSI outcome.colleagueWinter2009Royan Institute & IsfahanUniversity16 Elimination of Fas+ PI- (human) sperms via complement mediated lysis colleague Winter2009Royan Institute & EsfahanUniversity of Medical Sciences17 The study of relationship between epigenetic changes and semen colleague Winter Royan Institute & Esfahan

parameters, DNA fragmentation, protamine deficiency in ICSI and IVFpatients2008 University of Medical Sciences8) Prize:No TITLE AUTHOR STATUS DATE1Evaluation of sperm chromatin structure onfertilityTavalaee, Nasr-EsfahaniThe Winner of Hamandishi research festivalon medical sciences 20072Evaluation of sperm chromatin structure onfertilityTavalaee, Nasr-EsfahaniJavan Kharazmi research festival onmedical sciences20083As excellent researcher in Isfahan cityTavalaeePaghoheshi va fan avari festival20099) Presentation of Workshop:2 nd Royan Workshop on: Evaluaon of sperm funconal tests in Idencaon and treatment of inferle individuals. 2007; Royan Institute(Isfahan campus).3 nd Royan Workshop on: Evaluation of sperm functional tests in Identification and treatment of inferle individuals. 2009; Royan Instute(Isfahan campus).

1 nd 3nd Royan Workshop on: spermogram and its interpretaon in ART. 2010; Royan Instute (Isfahan campus).10) Consulting of thesis:1- Mahsa Kheirollahi. Sperm selection based on its binding ability to hyaluronic acid for intra cytoplasmic sperm injection. Isfahan University.2- Nazem Ghasemi. Comparison of sperm morphology, DNA fragmentaon, protamine deciency and HSPA2 expression before, three and sixmonth after varicocelectomy. Qazvin University of Medical Sciences.3- Zohre Fathi. The role of basal bodies on evaluation of protamine deficiency with flowcytometry.4- Masumeh Zareii Kheirabadi. The comparison of efficiency of DGC and DGC-Zeta for selection of sperm with low apoptosis.11) Research Prole (preview):A) Semen AnalysisB) Sperm functional testes:B-1) Nuclear chromatin maturity tests:- Chromomycin A3 staining (CMA3)- Aniline blue staining (Ab)- Aniline blue-Eosin staining (Ab - E)- Sodium Dodecyl Sulfate (SDS)

- Acridine orange staining (AO)- Sperm chromatin Dispersion (SCD)- Single cell gel electrophoresis (Comet assay)- DNA terminal deoxynucleotidyl transferase-mediated dUDP nick-end labelling (TUNEL assay)B-2) Sperm functional tests:-Hypo-Osmotic Swelling Test (HOST)- Gelatinolysis Test as indicator of acrosin activityC) Sperm selection proceduresC-1) Roune sperm selection procedures- Swim-up- Density gradient centrifugation (DGC)C-2) Novel sperm selection procedures- Hyaluronic (HA) binding method based on HA receptor

- Zeta method based on sperm surface charges- Combined density gradient and Zeta method- MACS method based on apoptotic marker12) Book publications:1- Sperm: Idencaon and selecon of sperm from Sperm the biological and clinical aspect in ICSI paents (2010), is written by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. ISBN: 978-964-8115-83-3.2- WHO laboratory manual for the Examination and processing of human semen, Translated by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee(ISBN: 978-600-6040-00-4).