The role of Formyl peptide receptor 2 (Fpr2) in leukocytes trafficking

The role of Formyl peptide receptor 2(Fpr2) in leukocytes traffickingDr. Vincenzo BrancaleoneWilliam Harvey Research InstituteBart’s and London School of Medicine and DentistryQueen Mary University of LondonCopenaghen, 22 nd July 2010

FPRs receptorsHumanMouseFPR1FPR2/ALXFPR376%96%83%Fpr1Fpr-rs1Fpr-rs2Fpr2Fpr-rs3Fpr-rs4Fpr-rs5Fpr-rs6Fpr-rs7

Resolution of inflammation• Resolution of inflammation is a relatively novel concept thatrepresents the response of innate immunity to inflammatory stimuli

Mediators and receptors involved ininflammatory responseN?AnnexinA1Lipoxin A4SAACpd 43FPR2G proteinC

Aim of the projectEstablish the role of FPR2 receptor ininflammatory events and in the resolutionof inflammation

In vivo methods• Fpr2 -/- mice colony and age matched wild type (+/+) controlmice– I) Intravital microscopy of mesenteric circulation bychallenging mice with ischemia-reperfusion protocol (I/R)(30’-90’ mins)– II ) Carrageenan-induced paw edema

Results – IVM+/+ sham -/- sham25um25um-/- I/R+/+ I/R25um25um

Results – IVMI/R 30’/90’, n=4-6+/+

Results – IVMI/R 30’/90’, n=4-6**** vs +/+

Results – IVM§§§§ vs sham* vs +/+§§***§§§§ § ***§§Sham Ischaemia I/RSham Ischaemia I/R

In vivo methods• Fpr2 -/- mice colony and age matched wild type (+/+) controlmice– I) Intravital microscopy of mesenteric circulation bychallenging mice with ischemia-reperfusion protocol(30’-90’ mins)– II ) Carrageenan-induced paw edema

Results – Paw oedemaFpr2 +/+Fpr2 -/-1% carrageenan2-192 hrs

Results – Paw oedema+/+-/-n=15

Results – Paw oedema+/+-/-n=15

Results – Paw oedemaHE staining left paw at 6hn=15+/+ -/-

Acknowledgements• Prof. Mauro Perretti• Prof. Rod Flower• Dr. Neil Dufton• Mr. Asif IqbalFunded by: