In Vitro Callus Induction from Stem Explants of Cissus ...
In Vitro Callus Induction from Stem Explants of Cissus ...
In Vitro Callus Induction from Stem Explants of Cissus ...
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
<strong>In</strong> <strong>Vitro</strong> …Kalpana Teware et al.Table: 1- Responses <strong>of</strong> callusing <strong>of</strong> <strong>Cissus</strong> quadrangularis L. in different month <strong>of</strong> year.S.NO. Months <strong>Callus</strong> induction1 Jan – Feb ++2 Mar-April +3 May-June ++++4 July- Aug ++++++5 Sep_Oct. +6 Nov- Dec. +Table: 2- Effect <strong>of</strong> various level and combinations <strong>of</strong> PGR’s for callusing <strong>of</strong> <strong>Cissus</strong>quadrangularis L.S.No. MS-Medium 2,4,D NAA Type <strong>of</strong> the callus Responses in%1 CM1 0.5 - No callus induction 0%2 CM2 1.0 - Dark brown friable callus 25%3 CM3 1.5. - Brown friable callus 35%4 CM4 2.0 - Light brown friable callus 50%5 CM5 2.5 - Light brown friable callus 65%6 CM6 3.0 - Slimy light brown friable callus 85%7 CM7 3.5. - Brown non friable callus 90%8 CM8 4.0 - Brown non friable callus 92%9 CM9 - 0.5 Light brown non friable callus 35%10 CM10 - 1.0 Light green non friable callus 40%11 CM11 1.5 Light brown whitish callus 50%12 CM12 2.0 Light green whitish callus 50%13 CM13 2.5 Light brown whitish callus 50%14 CM14 3.0 Light brown white non friable 65%callus15 CM15 3.5 Light brown white callus 70%16 CM16 4.0 Light brown non friable callus 75%1270 J. Chem. Bio. Phy. Sci. Sec. A, 2013, Vol.3, No.2, 1268-1272.
<strong>In</strong> <strong>Vitro</strong> …Kalpana Teware et al.Fig. 1: Responses <strong>of</strong> <strong>Callus</strong>ing <strong>In</strong> Different Month <strong>of</strong> The Year (2009-10) for <strong>Cissus</strong>quadrangularis L. HadjodFig. 2: Responses <strong>of</strong> <strong>Callus</strong>ing with Different PGR’s with MS Medium in<strong>Cissus</strong> quadrangularis L. (Hadjod)Fig. 3: <strong>Callus</strong> responses <strong>of</strong> <strong>Cissus</strong> quadrangularis L. with MSMedium <strong>of</strong> different composition.1271 J. Chem. Bio. Phy. Sci. Sec. A, 2013, Vol.3, No.2, 1268-1272.
<strong>In</strong> <strong>Vitro</strong> …Kalpana Teware et al.REFERENCES1. A.W.Panthong, D. Supraditaporn,T. Kanjanapothi, Taesotikul and V. Reutrakul. An analgesic,anti-inflammatory and venotonic effect <strong>of</strong> <strong>Cissus</strong> quadrangular is Linn. J. Ethnopharmacol,2007, 110: 264-270.2. S.P.Sen, Studies on the active constituents <strong>of</strong> <strong>Cissus</strong> quadrangularis L. Wall. Curr. Sci,1966, 35:317.3. K.K.Bhutani,R. Kapoor, C.K. Atal, Two unsymmetrical Tetracyclic triterpenoids <strong>from</strong> <strong>Cissus</strong>quadrangularis L .Phytochemistry,1984,23 (2),407.4. Anuj K.Srivastava1, Priyanka Shrivastava, B.R. Behera, A.K. Shrivastava,; Pharmacognostical& Phyto-Chemical <strong>In</strong>vestigation <strong>of</strong> <strong>Cissus</strong> quadrangularis Linn. <strong>Stem</strong>, 2011, 3, 020, 974–9446.5. T.Murashige, and I. A. Skoog, Physiole plant, 1962, 16:473-497.6. Mahendra Peenu, Singh Pratibha and Khan Shagufta.<strong>In</strong> vitr regeneration studies <strong>of</strong>Withania sominifera (L.) Dunal. <strong>In</strong>dian J. <strong>of</strong> Applied and pure Biology, 2004, 19(3):359-362.7. Khan, N. Alam, M.S. Nath; <strong>In</strong> <strong>Vitro</strong> regeneration <strong>of</strong> Garlic trough callus culture. J. <strong>of</strong>Biological sci. U.K. 2004,8. Datta Anirudha , Narula Alka,K.C. Banali, P.S. Shrivastava, Enhance alkaloid production incallus culture <strong>of</strong> liotropium indicu L. and regeneration <strong>of</strong> plantlets J. <strong>of</strong> Plant BiotechnologBiochemistry,2003,12: 139-142.9. Geta Rani , G.S.Virk, and Nagpal Avinash,; callus induction and plantlets regeneration onWithenia sominifera L. in vitro cellular anddevelopmental biology plant,2003, 39(5) : 468-474.Corresponding author: Teware Kalpana; Department <strong>of</strong> Bioscience SAM GirlsP.G. College, Bhopal(M.P.) <strong>In</strong>dia1272 J. Chem. Bio. Phy. Sci. Sec. A, 2013, Vol.3, No.2, 1268-1272.